We narrowed to 11,951 results for: emb
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Plasmid#147501PurposeMammalian Expression of HsNot2-F502ADepositorInsertHsNot2-F502A (CNOT2 Human)
ExpressionMammalianAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF194 Mamu-E*02:04
Plasmid#180461PurposeE. coli expression of soluble MHC proteinDepositorInsertMamu-E*02:04
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
lacI-Ptac-lacO-IMBB4-pUC
Plasmid#170770Purposetest plasmid for 5RM assembly, expresses lacI repressorDepositorInsertlacI
UseSynthetic BiologyPromoterlacI promoterAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBN089
Plasmid#173451Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y717E, Y718EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN095
Plasmid#173765Purposeexpress E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncationDepositorInsertE. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
Tagshexa-histidine tagExpressionBacterialMutationdelete motif 3PromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN083
Plasmid#173445Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y705E, Y706EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj15
Plasmid#173145PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj15 (kcnj15 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E34: attB4-mCMVp>-attB1r
Plasmid#162926PurposeGateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoterDepositorInsertminimal CMV IE1 promoter
UseSynthetic BiologyAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_dPKA-CAT1N17-mVenus
Plasmid#168497PurposeMammalian expression of the N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus.DepositorInsertN-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CβN15-mVenus
Plasmid#168496PurposeMammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus.DepositorInsertN-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E25: attB4-mPol2p>-attB5
Plasmid#162981PurposeGateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoterDepositorInsertmurine RNA polymerase 2 promoter
UseSynthetic BiologyAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C232-E34: attB2r-iRFP670-attB3
Plasmid#162908PurposeGateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteinsDepositorInsertfar-red iRFP670 fluorescent protein
UseSynthetic BiologyAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E19: attB4-mPol2p>-attB1r
Plasmid#162971PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInsertmurine RNA polymerase 2 promoter
UseSynthetic BiologyAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E26: attB4-SV40p>-attB1r
Plasmid#162923PurposeGateway attB4/attB1r entry clone for mammalian expression using the SV40 promoterDepositorInsertSV40 immediate early promoter
UseSynthetic BiologyAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 NtSPrbcS (GB2213)
Plasmid#160589PurposeB3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chainDepositorInsertNtSPrbcS
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 CUP2 (GB2716)
Plasmid#160599PurposeCopper dependent transcription factorDepositorInsertCUP2
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HiGlpG- S116A, H196A
Plasmid#158539PurposeBacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196ADepositorInsertHiGlpG
TagsGST tagExpressionBacterialMutationS116A;H169AAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-delN-EcGlpG
Plasmid#158537PurposeBacterial expression of rhomboid protease EcGlpG without N terminusDepositorInsertdelta N-terminus EcGlpG
TagsGST tagExpressionBacterialMutationN-terminal deletion (1-86)Available sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTV-mNanog-tdiRFP670-PEST
Plasmid#122347PurposeTargeting vector for mouse Nanog to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.DepositorInsert2A-tdiRFP-PEST with homology arms for mouse Nanog
UseMouse TargetingAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTV-mSlc2a3-tdiRFP670-PEST
Plasmid#122348PurposeTargeting vector for mouse Slc2a3 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.DepositorInsert2A-tdiRFP-PEST with homology arms for mouse Slc2a3
UseMouse TargetingAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only