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pJH25_pBad_AraC_Para-sfGFP.0TAG

Plasmid

#163720

Purpose

Arabinose-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG GLTP

Plasmid

#170740

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Insert

GLTP (GLTP Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMM549

Plasmid

#112531

Purpose

pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA

Expression

Bacterial

Promoter

J23107 and ProD

Available Since

Feb. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSK234

Plasmid

#131149

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Hiv7CMV-Cremyc-2A-GFP

Plasmid

#117148

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Use

Lentiviral

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-clbC

Plasmid

#114145

Purpose

Expresses ClbC-nHis

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbC

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-clbBpks

Plasmid

#114154

Purpose

Expresses the PKS module of ClbB

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbB

Tags

6xHis

Expression

Bacterial

Mutation

Only contains the PKS module of ClbB

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG545

Plasmid

#105424

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

AvrBs3

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAP576-2

Plasmid

#99481

Purpose

3XFlag::tagBFP::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagBFP::Myc

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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371-Bc

Plasmid

#97028

Purpose

yORF-tev-His6

Depositor

Chris Jeans

Type

Empty backbone

Tags

His6 tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S111A

Plasmid

#85619

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S111A

Tags

V5

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S250A

Plasmid

#85618

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S250A

Tags

V5

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pNXRVat-HACre

Plasmid

#78399

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
TKanAX

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a-Myc13

Plasmid

#78360

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

13xMyc
kanMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3b-PK9

Plasmid

#78363

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

9xPK
hygMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3b-PK12

Plasmid

#78364

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

12xPK
hygMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSuper 44-2

Plasmid

#51598

Purpose

shRNA 2 against rat Sema4D

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

shRNA2

Use

RNAi

Promoter

U6

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pICH77901

Plasmid

#50341

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, mas (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH77911

Plasmid

#50342

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ags (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH71431

Plasmid

#50344

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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