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pCambia2300 - ProRUBY:RUBY-Citrine

Plasmid

#128796

Purpose

Fluorescent reporter for subcellular localisation and expression analysis of RUBY

Depositor

George Haughn

Article

Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

Insert

RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)

Tags

Citrine

Expression

Plant

Promoter

ProRUBY

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP.N1-HDAC6.delta Buz

Plasmid

#36190

Depositor

Tso-Pang Yao

Article

Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

Insert

HDAC6..delta Buz (HDAC6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the C-terminal ub binding domain (BUZ…

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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ins:MCS2; cryaa:RFP

Plasmid

#110286

Purpose

A zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Type

Empty backbone

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pPBC-G6s

Plasmid

#62812

Purpose

pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6s-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-CCSS

Plasmid

#83424

Purpose

Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

Promoter

CMV

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pETM33_CRK_SH3

Plasmid

#178472

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CRK_SH3 (CRK Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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mRFP-mem

Plasmid

#55622

Purpose

This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

mRFP-Mem

Tags

The N-terminal 20 residues of neuromodulin are fu…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-53BP1LCD

Plasmid

#204407

Purpose

E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

53BP1(1208-1977) (TP53BP1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_ELAVL1

Plasmid

#106104

Purpose

Inducible gene expression of FlagHA ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ELAVL1 (ELAVL1 Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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LV-Let7d-5p

Plasmid

#117055

Purpose

Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

Let-7d-5p - OFP

Use

Lentiviral

Promoter

SFFV promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBX_RPL7a_smRuby2_HA

Plasmid

#236087

Purpose

Constitutively encodes RPL7a-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting the 60S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPL7a-smRuby2-HA (RPL7A Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(2)-CRY2-mCherry

Plasmid

#160441

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Twitch-3 pcDNA3

Plasmid

#49532

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pJM019

Plasmid

#134158

Purpose

Retroviral plasmid expressing GFP PSMA3

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

PSMA3 (PSMA3 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p207-Switch-ON (FRT)

Plasmid

#217886

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-hIFITM3-Y20A

Plasmid

#58462

Purpose

Expresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 to alanine

Promoter

CMV IE

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
NSP6-FR-2Strep

Plasmid

#210317

Purpose

Expresses SARS-CoV-2 NSP6 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PB-Hyg-Dest-mCherry-PAR-6B

Plasmid

#194908

Purpose

To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

PAR-6B (Pard6b Mouse)

Use

Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

39 C to G, silent mutation

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFX-Pickles 2.34NES

Plasmid

#100028

Purpose

Improved FRET biosensor for the measurement of BCR-ABL activity.

Depositor

Yusuke Ohba

Article

Horiguchi et al Cell Struct Funct. 2017 Feb 2;42(1):15-2

Insert

Pickles-2.34NES

Use

Pfx vector is derived from pcmv vector.

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR223-PLXNB2

Plasmid

#86236

Purpose

PLXNB2 full-length human cDNA CDS in gateway entry plasmid

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

PLXNB2 (PLXNB2 Human)

Use

Gateway entry vector

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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