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pT2U3-YFP

Plasmid

#84547

Purpose

Hybrid reporter construct with 5 transcription factor binding sites (2 TetO, 3 UAS) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U3-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T4-YFP

Plasmid

#84548

Purpose

Hybrid reporter construct with 6 transcription factor binding sites (2 UAS, 4 TetO) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T4-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U2T2-YFP

Plasmid

#84549

Purpose

Hybrid reporter construct with 6 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2T2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T2U2T2-YFP

Plasmid

#84550

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 2 TetO, 2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T2U2T2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pU2T4U2-YFP

Plasmid

#84551

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 UAS, 4 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T4U2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U2T2U2-YFP

Plasmid

#84552

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO, 2 UAS) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U2T2U2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT2U4T2-YFP

Plasmid

#84553

Purpose

Hybrid reporter construct with 8 transcription factor binding sites (2 TetO, 4 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

T2U4T2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU2T2-YFP

Plasmid

#84543

Purpose

Hybrid reporter construct with 4 transcription factor binding sites (2 UAS, 2 TetO) driving EYFP

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

U2T2-EYFP

Expression

Mammalian

Promoter

CMV-min

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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OA-986C

Plasmid

#125000

Purpose

Expresses dCas9-VPR driven by the bottleneck promoter

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

bottleneck

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986D

Plasmid

#125001

Purpose

Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Expression

Insect

Promoter

ubiquitin

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986E

Plasmid

#125002

Purpose

Expresses dCas9-VP64 driven by the bottleneck promoter

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Expression

Insect

Promoter

bottleneck

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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IDA3::HA exon2 (WS13)

Plasmid

#126874

Purpose

p3xHA inserted within second exon of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Publication

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pExcisionase-AntiDsRed

Plasmid

#139675

Purpose

Cassette for testing phiC31 Excisionase activity using a fluorescent reporter

Depositor

James Birchler

Publication

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed phiC31 Excisionase

Expression

Plant

Promoter

Maize Ubiquitin

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK224

Plasmid

#110590

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pC0.171

Plasmid

#119639

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS4 Up Flank

Use

Synthetic Biology

Mutation

A691T in NS4 Up Flank

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.057

Plasmid

#119565

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ParsB

Use

Synthetic Biology

Mutation

bp 117 G to A, RBS* added (22 bp) upstream of ATG.

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtB

Plasmid

#114344

Purpose

crtB in pStA0

Depositor

John Heap

Publication

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtB (codon optimised)

Use

Synthetic Biology

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::dxs

Plasmid

#114346

Purpose

dxs in pStA0

Depositor

John Heap

Publication

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

dxs (codon optimised)

Use

Synthetic Biology

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtZ

Plasmid

#114348

Purpose

crtZ in pStA0

Depositor

John Heap

Publication

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtZ (codon optimised)

Use

Synthetic Biology

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::lcyB

Plasmid

#114349

Purpose

lcyB in pStA0

Depositor

John Heap

Publication

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

lcyB (codon optimised)

Use

Synthetic Biology

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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