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LentiU6-hMYOD1 gRNA_1-MS2-Puro

Plasmid

#192673

Purpose

Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human MYOD1 activating gRNA #1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-CPZ-TAN3 vpr

Plasmid

#115833

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-TAN3 Vpr (DQ374658)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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p6899 PHAGE-P CMVT N-HA-hE6AP II C843A

Plasmid

#37604

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II C843A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C843A

Promoter

CMVt

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
mNeonGreen-Sec24D

Plasmid

#226581

Purpose

Encodes Sec24D protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

Insert

Sec24D (Sec24 homolog D) (SEC24D Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-CPZ-LB7 vpr

Plasmid

#115834

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-LB7 Vpr (DQ373064)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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A3Bi-Cas9n-UGI

Plasmid

#119135

Purpose

Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3B L1 intron-Cas9 nickase-UGI

Use

CRISPR

Mutation

L1 intron insertion

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBS mouse c-Myc

Plasmid

#14972

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (Myc Mouse)

Expression

Bacterial

Mutation

Nucleotides 2578-3294

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

Plasmid

#139649

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

H2B-mCherry

Use

Lentiviral

Tags

FLAG, His6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFlox_N-p300_BSD_FKBPF36V_EYFP

Plasmid

#163885

Purpose

Acute Depletion P300-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Ep300 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

Plasmid

#182826

Purpose

Cre-dependent expression of membrane localized APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

LckAPEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts1

Plasmid

#115886

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pIC292

Plasmid

#51405

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Article

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska2 IMAGE 4685972 (SKA2 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GOR -vpr

Plasmid

#115835

Purpose

Encodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGOR Vpr (FJ424871)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER IKKalpha

Plasmid

#26208

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK alpha (CHUK Human)

Use

RNAi

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pILGFP95

Plasmid

#83550

Purpose

Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

PDA1 promoter-yEGFP

Expression

Yeast

Promoter

PDA1

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only
Flag Nprl2 pLJM1

Plasmid

#46338

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_SNX18-1/1

Plasmid

#91369

Purpose

Protein expression and purification of human SH3 domain construct SNX18-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX18-1/1 (SNX18 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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polyPRM5

Plasmid

#175232

Purpose

Bacterial expression and purification, mixed with polySH3 to form condensates

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

ABL1 (ABL1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLuciferase (GB0096)

Plasmid

#68201

Purpose

Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luciferase coding region

Use

Luciferase and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_BIRmut

Plasmid

#197968

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_BIRmut

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

C328S, C331S, L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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