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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,901 results for: cloning

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Showing: 10161 - 10180 of 63901 results

  1. pLyGo-Ec-8

    Plasmid
    #163136
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Publication
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    P(rhaB)
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    163136-plasmid-map-sequence-id-332138

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  2. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139087
    Purpose
    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139087-plasmid-map-sequence-id-273883

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  7. pRDA_887

    Plasmid
    #245329
    Purpose
    Cas12a CRISPRa cloning vector; contains NbALFA, p65
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245329-plasmid-map-sequence-id-488027

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Showing: 10161 - 10180 of 63901 results