We narrowed to 1,213 results for: Aspa

1. pSP64T acvr1ba*

   Plasmid

   #185409

   Purpose

   Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   acvr1ba (acvr1ba Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 206 to Aspartic acid

   Promoter

   Available since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-Rab8a-TN

   Plasmid

   #86080

   Purpose

   A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed threonine 22 to aspargine

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. RF-118-UB-BRCA1

   Plasmid

   #78960

   Purpose

   Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells

   Depositor

   Richard Fahlman

   Article

   Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   3x Flag and ubiquitin

   Expression

   Mammalian

   Mutation

   delta 1-1155

   Promoter

   CMV

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMALp2x-SCO5461(E164D)(43-204)

   Plasmid

   #42508

   Depositor

   Koji Okamoto

   Article

   Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

   Insert

   guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
   

   Use

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-42 (nucleotide 1-126); chan…

   Promoter

   tac

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pTG-mTDGa.1

   Plasmid

   #81049

   Purpose

   bacterial expression of catalytically dead murine TDG

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TDG (Tdg Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   asparagine 151 changed to alanine

   Promoter

   T7

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CITED2-T166N

   Plasmid

   #47749

   Purpose

   Introduces a T166N point mutation in the CITED2 gene

   Depositor

   Shoumo Bhattacharya

   Article

   Chen et al PLoS One. 2012;7(10):e46256. doi: 10.137

   Insert

   CITED2 (CITED2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Threonine 166 to Asparagine

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611

   Plasmid

   #228374

   Purpose

   Expresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localization

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91
   

   Use

   Tags

   Green Florescent Protien

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGEX-parkin K161N

   Plasmid

   #227365

   Purpose

   For bacterial expression of parkin with a mutated pUbl/pUb binding site on RING0.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin K161N (PRKN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJExpress401-KDAC8 D101R

   Plasmid

   #224349

   Purpose

   Expresses human KDAC8 (HDAC8) D101R in E. coli

   Depositor

   Terry Watt

   Article

   Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

   Insert

   KDAC8 (HDAC8 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Bacterial

   Mutation

   Aspartate 101 mutated to arginine

   Promoter

   T5

   Available since

   Oct. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pJExpress401-KDAC8 D101A

    Plasmid

    #224347

    Purpose

    Expresses human KDAC8 (HDAC8) D101A in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Aspartate 101 mutated to alanine

    Promoter

    T5

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3-RIPK3[V458D][Q459D][V460D][G461D]-FLAG

    Plasmid

    #199327

    Purpose

    mutated RHIM motive (interaction active)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed V458, Q459,V460 and G461 to aspartic acid

    Promoter

    Available since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. DNMT2Δ191-237

    Plasmid

    #198382

    Purpose

    Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

    Insert

    DNMT2 (TRDMT1 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 191-237

    Promoter

    T7

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sandgrouse+R111Q–insD

    Plasmid

    #191229

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    removed aspartic acid at 121 and replaced arginin…

    Promoter

    PH and p10

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS313_ATP3mut

    Plasmid

    #120256

    Purpose

    Expression of ATP3-67 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 304 changed to Asparagine; Alanine 307…

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET151-GST-PP-RPN13 (19-132) R104D

    Plasmid

    #125827

    Purpose

    Expresses GST-RPN13 (19-132) R104D in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to aspartate

    Promoter

    T7

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCu-GFP-Atg17(416) (L313D I314K)

    Plasmid

    #121409

    Purpose

    Expressing GFP-Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-GFP-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 313 to Aspartic acid; Isoleucine 314 to …

    Promoter

    CUP1 promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

    Plasmid

    #106462

    Purpose

    Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W(N163,242Q)
    

    Use

    Tags

    PA

    Expression

    Bacterial and Yeast

    Mutation

    changed Asparagines at positions 163 and 242 to G…

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

    Plasmid

    #106468

    Purpose

    Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W(D415A)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Changed Aspartate 415 to Alanine

    Promoter

    YBR139W

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-20b-T7-Ntx-K49

    Plasmid

    #58715

    Purpose

    Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    T7 peptide tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic Acid 49 to Lysine

    Promoter

    T7

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid

    #58490

    Purpose

    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

    Insert

    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Aspartic Acid 95 to Glutamine

    Promoter

    Ubiquitin

    Available since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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