We narrowed to 13,364 results for: Kars;

1. pLKO-RFP-shCntrl

   Plasmid

   #69040

   Purpose

   Lentiviral expression of control shRNA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   control shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pACλCI-β-flap (831-1057)

   Plasmid

   #53733

   Purpose

   Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   λCI-β-flap fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac UV5

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Beta-Lactamase

   Plasmid

   #62729

   Purpose

   Expresses Beta-Lactamase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Beta-Lactamase
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Deletion of periplasmic signal domain

   Promoter

   T7

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pASTA3

   Plasmid

   #24657

   Depositor

   Tanya Parish

   Article

   Carroll et al PLoS One. 2010 . 5(3):e9823.

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-NHERF1

   Plasmid

   #31650

   Depositor

   Edward Weinman

   Article

   Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

   Insert

   solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
   

   Use

   Tags

   His & S tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pME-Cas9

   Plasmid

   #64237

   Purpose

   expresses codon optimized Cas9 in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   codon optimized Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-ECFPEYFP

   Plasmid

   #24520

   Depositor

   Liusheng He, Jehonathan Pinthus

   Article

   He et al Cytometry A. 2003 Oct . 55(2):71-85.

   Insert

   ECFP, EYFP
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pBRα

   Plasmid

   #53731

   Purpose

   Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   αNTD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6a:sgRNA(tyr)

   Plasmid

   #64250

   Purpose

   expresses sgRNA(tyr) under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   U6a:sgRNA (tyr)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DHFR-myc

    Plasmid

    #20214

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCHARGE3

    Plasmid

    #24658

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    Turbo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-cdc2-AF (718)

    Plasmid

    #39872

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    Cdc2 (CDK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AF = T14A, Y15F

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

13. MDH1-PGK-H2Kb2

    Plasmid

    #17852

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    H-2Kb (H2-K1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. V309 - pDNR MCS SA lacZ alpha - Donor

    Plasmid

    #11692

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. FUW-TetO-Wt1

    Plasmid

    #41082

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Wt1 (-KTS alternative isoform) (Wt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO + minimal CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCellFree_G03

    Plasmid

    #67137

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis eGFP tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-6P-1 Stu2 1-306

    Plasmid

    #38315

    Depositor

    Anthony Hyman

    Article

    Widlund et al Mol Biol Cell. 2012 Sep 19.

    Insert

    5' 918 bp from STU2 ORF (STU2 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV_dCas9_VP64

    Plasmid

    #49015

    Purpose

    encodes human-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (human-codon-optimized)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FRB-myc

    Plasmid

    #20228

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. DdMCU

    Plasmid

    #67642

    Purpose

    DdMCU-Flag in pACT2 vector human codon optimised

    Depositor

    Vamsi Mootha

    Article

    Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    DdMCU
    

    Use

    Tags

    Flag tag with linker

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge