Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,650 results for: LRE

Showing: 1001 - 1020 of 3650 results

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CDKL1 gRNA (BRDN0001148261)

Plasmid

#76270

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDKL1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDKL1 (CDKL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001146337)

Plasmid

#75707

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001146285)

Plasmid

#75708

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001147842)

Plasmid

#75709

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK20 gRNA (BRDN0001144911)

Plasmid

#75706

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK20

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK20 (CDK20 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

Plasmid

#67977

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

Plasmid

#67974

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2ABFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-Cas9Bsd-W

Plasmid

#68343

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-66Q-myc-WPRE

Plasmid

#107934

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)

Use

AAV

Promoter

hSynapsin

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCSC-ISL1-T2A-LHX3

Plasmid

#90215

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

ISL1-T2A-LHX3 (ISL1, LHX3 Human)

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-BsdCas9-W

Plasmid

#67978

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-Htt171-18Q-myc-WPRE

Plasmid

#107936

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of wild type HTT (HTT Human)

Use

AAV

Promoter

hSynapsin

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72666

Purpose

Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

Plasmid

#71707

Purpose

A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

Depositor

Giulio Draetta

Article

Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

Insert

humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)

Use

CRISPR

Tags

3xFLAG and hGEM(1/110)

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

Plasmid

#67991

Purpose

CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Showing: 1001 - 1020 of 3650 results

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