We narrowed to 11,396 results for: Vars;

1. pCSJ392

   Plasmid

   #159722

   Purpose

   Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   GID10 (GID10 Budding Yeast)
   

   Use

   Yeast two-hybrid assay

   Tags

   3xflag and Gal4AD

   Expression

   Yeast

   Mutation

   remove stop codon

   Promoter

   ADH1

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCellFree_KA1

   Plasmid

   #145369

   Purpose

   Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Type

   Empty backbone

   Use

   Tags

   His and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1P shNFS1_4

   Plasmid

   #102966

   Purpose

   Suppression of NFS1

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shNFS1_4 (NFS1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9 (BB)-2A-GFP gamma-tubulin sg

   Plasmid

   #104437

   Purpose

   Knockdown the expression of gamma-tubulin in human cells

   Depositor

   Maria Alvarado-Kristensson

   Article

   Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

   Insert

   gamma-tubulin sgRNA
   

   Use

   Tags

   The gamma-tubulin sgRNA is coexpressed with 3xFLA…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1P shNFS1_3

   Plasmid

   #102965

   Purpose

   Suppression of NFS1

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shNFS1_3 (NFS1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. ReNL-Actin_pcDNA3

   Plasmid

   #89531

   Purpose

   Red color variant of bright luminescent protein, fused to actin

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Use

   Tags

   Actin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. tubulin-OeNL_pcDNA3

   Plasmid

   #89523

   Purpose

   Orange color variant of bright luminescent protein, fused to b-tubulin

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Use

   Tags

   b-tubulin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL-RNAINS4

   Plasmid

   #69456

   Purpose

   RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S4 + SFGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   mutations for S4 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL-RNAINS3

   Plasmid

   #69455

   Purpose

   RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   mutations for S3 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNB762

    Plasmid

    #60150

    Purpose

    Firefly luciferase (Promega) driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNB767

    Plasmid

    #60159

    Purpose

    Firefly luciferase (Promega) driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNB649

    Plasmid

    #60136

    Purpose

    Firefly luciferase (Promega) driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSUPER.retro.puro-THAP11-A

    Plasmid

    #36993

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 shRNA (THAP11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSUPER.retro.puro-THAP11-C

    Plasmid

    #36994

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 shRNA (THAP11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pSUPER.retro.puro-THAP11-E

    Plasmid

    #36995

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 shRNA (THAP11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pHAGE-mt-mKeima

    Plasmid

    #131626

    Purpose

    lentiviral expression of mitochondrial mKeima for generation of stable cell lines

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    mt-mKeima
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC-tag-N

    Plasmid

    #108078

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/BioID

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CCDB (ccdB(letD) )
    

    Use

    Tags

    StrepIII-HA-BirA*

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-RLuc8.6-535

    Plasmid

    #87125

    Purpose

    Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

    Depositor

    Sanjiv Sam Gambhir

    Article

    Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

    Insert

    Renilla Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-DAG1

    Plasmid

    #205149

    Purpose

    Overexpress DAG1

    Depositor

    Monkol Lek

    Insert

    Dystroglycan 1 (DAG1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Carries common missense variant c.41C>G (p.Ser…

    Promoter

    EF-1a

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. LZRS-DNMT1

    Plasmid

    #24952

    Depositor

    Paul Khavari

    Article

    Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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