Addgene

We narrowed to 23,703 results for: spr

Showing: 1001 - 1020 of 23703 results

Results per page:

Sort results by:

The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit

Plasmid Kit

#1000000210

Purpose

Toolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.

Depositor

Vincent Martin

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only
zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

Plasmid Kit

#1000000171

Purpose

Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

Depositor

Sylvestre Marillonnet, Johannes Stuttmann

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only
MG1655 Z1 malE

Bacterial Strain

#65915

Purpose

E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Bacterial Resistance

None

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
lentiCas9-Blast (Lentiviral Prep)

Viral Prep

#52962-LV

Purpose

Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Promoter

EFS-NS

Tags

None

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only
Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)

Viral Prep

#73178-LV

Purpose

Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Promoter

Tags

None

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only
Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

Viral Prep

#73633-LV

Purpose

Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p><p>Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.</p></p>

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Promoter

Tags

None

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only
MG1655 Z1 malE clpA

Bacterial Strain

#65918

Purpose

E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, clpA KO

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Bacterial Resistance

None

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
MG1655 Z1 malE aat

Bacterial Strain

#65917

Purpose

E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, aat KO

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Bacterial Resistance

None

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
UQ5875

Bacterial Strain

#37167

Depositor

Gary Roberts

Bacterial Resistance

Ampicillin and kanamycin

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
dCAS9-VP64_GFP (Concentrated Lentiviral Prep)

Viral Prep

#61422-LVC

Purpose

Ready-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Promoter

EF1a

Tags

None

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only
lenti dCAS-VP64_Blast (Lentiviral Prep)

Viral Prep

#61425-LV

Purpose

Ready-to-use Lentiviral Prep particles produced from lenti dCAS-VP64_Blast (#61425). In addition to the viral particles, you will also receive purified lenti dCAS-VP64_Blast plasmid DNA.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Promoter

EF1a

Tags

None

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only
UQ6139

Bacterial Strain

#37177

Depositor

Gary Roberts

Bacterial Resistance

Ampicillin and kanamycin

Species

Burkholderia xenovorans

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only
hSyn1-eYFP (AAV9)

Viral Prep

#117382-AAV9

Purpose

Ready-to-use AAV9 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

Syn

Tags

eYFP

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
hSyn1-eYFP (AAV5)

Viral Prep

#117382-AAV5

Purpose

Ready-to-use AAV5 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

Syn

Tags

eYFP

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only
hSyn1-eYFP (AAV2)

Viral Prep

#117382-AAV2

Purpose

Ready-to-use AAV2 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

Syn

Tags

eYFP

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only
hSyn1-eYFP (AAV8)

Viral Prep

#117382-AAV8

Purpose

Ready-to-use AAV8 particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

Syn

Tags

eYFP

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-eYFP (AAV5)

Viral Prep

#104055-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV-CAG-eYFP (#104055). In addition to the viral particles, you will also receive purified pAAV-CAG-eYFP plasmid DNA. CAG-driven eYFP expression control. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

CAG

Tags

EYFP

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-eYFP (AAV2)

Viral Prep

#104055-AAV2

Purpose

Ready-to-use AAV2 particles produced from pAAV-CAG-eYFP (#104055). In addition to the viral particles, you will also receive purified pAAV-CAG-eYFP plasmid DNA. CAG-driven eYFP expression control. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

CAG

Tags

EYFP

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only
TRE-DIO-eYFP (AAV1)

Viral Prep

#117383-AAV1

Purpose

Ready-to-use AAV1 particles produced from TRE-DIO-eYFP (#117383). In addition to the viral particles, you will also receive purified TRE-DIO-eYFP plasmid DNA. Tet-inducible, Cre-dependent eYFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

TRE

Tags

eYFP (Tet-inducible, Cre-dependent)

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
hSyn1-eYFP (AAV PHP.eB)

Viral Prep

#117382-PHPeB

Purpose

Ready-to-use AAV PHP.eB particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

Syn

Tags

eYFP

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Showing: 1001 - 1020 of 23703 results

Results per page:

Sort results by:

The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit

zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

MG1655 Z1 malE

lentiCas9-Blast (Lentiviral Prep)

Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)

Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

MG1655 Z1 malE clpA

MG1655 Z1 malE aat

UQ5875

dCAS9-VP64_GFP (Concentrated Lentiviral Prep)

lenti dCAS-VP64_Blast (Lentiviral Prep)

UQ6139

hSyn1-eYFP (AAV9)

hSyn1-eYFP (AAV5)

hSyn1-eYFP (AAV2)

hSyn1-eYFP (AAV8)

pAAV-CAG-eYFP (AAV5)

pAAV-CAG-eYFP (AAV2)

TRE-DIO-eYFP (AAV1)

hSyn1-eYFP (AAV PHP.eB)

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections

	More than 20 requests
	More than 50 requests
	More than 100 requests