We narrowed to 7,804 results for: ttl

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pDONR223 SARS-CoV-2 ORF8_nostop

Plasmid

#149326

Purpose

Gateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP5_nostop

Plasmid

#149308

Purpose

Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221 UBR5 ∆UBR

Plasmid

#81063

Purpose

Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)

Use

Cloning

Mutation

W1235L (bp g3704t)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆HECT

Plasmid

#81065

Purpose

Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)

Use

Cloning

Mutation

C2768A (bp t8302g and g8303c)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAUX_OL

Plasmid

#166246

Purpose

use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

Depositor

Domitilla Del Vecchio

Publication

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

[P112-sgRNA-term]-[J23116_B34-dCas9-B15]

Expression

Bacterial

Promoter

Ec-TTL-P112 and BBa_J23116

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163073

Purpose

Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mito-roGFP2-Grx1

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163076

Purpose

Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mito-roGFP2-Orp1

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-NPR2

Plasmid

#23479

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NPR2 (NPR2 Human)

Use

Gateway Cloning

Mutation

isoform missing aa905-962

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR-Cre

Plasmid

#139545

Purpose

Cre ORF with stop codon between attL1-attL2 on Gateway entry vector

Depositor

Minoru Ko

Insert

Cre

Use

Cre/Lox

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141B2.0

Plasmid

#99897

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ141D2.0

Plasmid

#99906

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENTR4-V5-2 (w234-1)

Plasmid

#17426

Purpose

Gateway entry vector for N-term V5 tag, frame B

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

V5

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEF 3-CFP-2

Plasmid

#20105

Depositor

Antonio Richart

Insert

CFP (Cfp )

Use

Synthetic Biology; Plant gateway vector

Available since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pME-mCherry-24xMBS

Plasmid

#86242

Purpose

Middle entry vector for generating MBS tagged RNAs with a fluorescent protein reporter

Depositor

Florence Marlow

Publication

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

mCherry-24xMBS

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2139

Plasmid

#74111

Purpose

Membrane targeted PH-miniSOG entry clone for efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Publication

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG

Use

Gateway topo cloning

Tags

PH

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E051 Hs.ECT2

Plasmid

#70335

Purpose

Gateway ORF clone of human ECT2 [NM_018098.5] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

ECT2 (ECT2 Human)

Use

Gateway Cloning

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Bsrs074-Plas

Plasmid

#85155

Purpose

Inducibile Las Promoter driving sfGFP

Depositor

Jeff Hasty

Publication

Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Plas-sfGFP

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pME-secAnxaV-NS

Plasmid

#67718

Purpose

Gateway entry clone containing secreted human AnxaV with no stop codon

Depositor

Rob Parton

Publication

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

secAnxaV-NS

Expression

Mammalian

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR L3-4XeGFP-Myc-L2

Plasmid

#32316

Depositor

Steve Stowers

Publication

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

4XeGFP-Myc

Use

Gateway multisite entry vector

Tags

Myc

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pME minprom_creERT2

Plasmid

#195966

Purpose

Gateway pME CreERT2 ORF

Depositor

Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter creERT2

Use

Cre/Lox

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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