We narrowed to 45,592 results for: cha;
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Plasmid#206033PurposeMammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoterDepositorInserthXRCC1
TagseGFPExpressionMammalianMutationS103A,R186A,S184A,S193A,S219A,S220A,S236A,S268APromoterCAGAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
TagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7Available SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR2_mScarlet
Plasmid#188039PurposeExpresses CapChR2-mScarlet in mammalian neuronsDepositorInsertCapChR2-mScarlet
UseAAVTagsmScarletMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/Ecad-tdTomato
Plasmid#101278PurposeLentiviral expression of shRNA targeting Ecad and Ecad-tdtomatoDepositorInsertE-cadherin
UseLentiviralTagstdtomatoExpressionMammalianAvailable SinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-CB2-HF
Plasmid#121682PurposeBacterial expression of cannabinoid receptor CB2 fusion proteinDepositorInsertperipheral cannabinoid receptor (CNR2 Human)
TagsHis6 Flag tagExpressionBacterialPromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin2
Plasmid#72595PurposeoverexpressionDepositorInsertSESN2 (SESN2 Human)
TagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-5
Plasmid#228965PurposeFor bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-5
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(mito-LD)
Plasmid#214421PurposeDetection of mito-lipid droplet contact siteDepositorInsertRspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-3XHA-V5PromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II SK--POLQ
Plasmid#64873Purposecloning vector for human POLQDepositorInsertPOLQ (POLQ Human)
ExpressionBacterialAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A
Plasmid#110555PurposepFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.DepositorInsertAnti-RD114 heavy chain
TagsmIgG2AExpressionBacterial and MammalianPromoterEF-1 alpha core promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sc++
Plasmid#155011PurposeExpresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cellsDepositorInsertSc++
ExpressionMammalianMutationT1227K, Loop Substitution from S. anginosusPromoterEF1ɑAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TSF-TEVcs-ULK1
Plasmid#171416PurposeFor the purifcation of human ULK1 complexDepositorInsertULK1 (ULK1 Synthetic)
ExpressionMammalianAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
MP28445 – pFUSE2ss-aRD114_CLIg-mIgK
Plasmid#110556PurposepFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.DepositorInsertanit-RD114 light chain
TagsmKappaExpressionBacterial and MammalianPromoterEF-1 alpha core promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
strep-IRF8
Plasmid#102872PurposeExpress strep-tagged mouse IRF8 protein in E. coliDepositorAvailable SinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE
Plasmid#161925PurposeLentiviral backbone for GoldenGate cloning effectors as a fusion with rTetRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyExpressionMammalianPromoterpEFAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-14
Plasmid#228994PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-14
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYP-GFP
Plasmid#197283PurposeEncodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.DepositorInsertSYP and GFP (Syp Rat)
UseLentiviralTags1D4 epitopeMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-DIO-tTA
Plasmid#99121PurposeAn AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTADepositorHas ServiceAAV1InsertDIO-tTA
UseAAVExpressionMammalianMutation*(see below)PromoterihSyn1Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR1_eYFP
Plasmid#188033PurposeExpresses CapChR1-eYFP in mammalian neuronsDepositorInsertCapChR1-eYFP
UseAAVTagseYFPMutationS63D, L132C, T159C and N258E mutations in CrChR2PromoterhSynAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Sestrin2
Plasmid#72593Purposeoverexpression, codon optimized for mammalian expressionDepositorInsertSESN2 (SESN2 Human)
TagsHAExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only