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pV1524

Plasmid

#111431

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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LZRS-HOTAIR

Plasmid

#26110

Depositor

Howard Chang

Article

Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

Insert

HOTAIR (HOTAIR Human)

Use

Retroviral

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PLD1

Plasmid

#45268

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PLD1 (PLD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag ERbeta

Plasmid

#35562

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 26, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-SP110C

Plasmid

#65393

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP110C

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pLenti.CMV/TO_PRKAA1

Plasmid

#74446

Purpose

Lentiviral expression vector encoding untagged WT human AMPK alpha 1

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

AMPK alpha 1 (PRKAA1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE hSPC-eGFP-W

Plasmid

#36450

Depositor

Darrell Kotton

Article

Longmire et al Cell Stem Cell. 2012 Apr 6;10(4):398-411

Inserts

human SP-C promoter
eGFP (eGFP )

Use

Lentiviral

Promoter

N/A and human SP-C promoter

Available Since

May 11, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-SP110A

Plasmid

#65392

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP110A

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

Plasmid

#84468

Purpose

pAAV-CRAM-tdTomato

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

tdTomato

Use

AAV

Mutation

T230I (please see depositor comments below)

Promoter

TRE

Available Since

Dec. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

Plasmid

#84471

Purpose

pAAV-RAM-ChR2

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Has Service

AAV8

Insert

Channelrhodopsin 2

Use

AAV

Tags

EYFP

Mutation

H134R

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

Plasmid

#84472

Purpose

pAAV-RAM-ArchT

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

ArchT

Use

AAV

Tags

EGFP

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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p6599 MSCV-IP N-HAonly FOSL1

Plasmid

#34897

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

FOSL1 (FOSL1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K29R

Plasmid

#17602

Purpose

Mammalian expression of HA tagged ubiquitin K29R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K29R; V5 deleted

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

Plasmid

#84467

Purpose

Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-Parkin

Plasmid

#17612

Depositor

Ted Dawson

Article

Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

Insert

Parkin (PRKN Human)

Tags

Myc

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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138-dCas9-Dnmt3a

Plasmid

#84570

Purpose

PiggyBac transposon system construct with dCas9-Dnmt3a

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Human, Synthetic)

Expression

Mammalian

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

Plasmid

#112005

Purpose

For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV5 and AAV9

Insert

axon-GCaMP6s-P2A-mRuby3

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK1

Plasmid

#78842

Purpose

To overexpress RIPK1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-FLEx-axon-GCaMP6s

Plasmid

#112010

Purpose

For cre-dependent enriched expression of GCaMP6s in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV5 and AAV9

Insert

axon-GCaMP6s

Use

AAV and Cre/Lox

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

Plasmid

#85146

Purpose

lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mRuby3-P2A-mTurquoise-MLC

Use

Lentiviral

Tags

mRuby3, mTurquoise

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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