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4LNDDE488Q-pCDNA3.1(+)

Plasmid

#172228

Purpose

Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

Depositor

Joshua Rosenthal

Article

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

Lambda N peptide - human ADAR2 Deaminase Domain E488Q

Tags

6xHis and Flag

Expression

Mammalian

Mutation

changed the Glutamic acid 488 in the Deaminase Do…

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcmvtag2Bmciap2 mut

Plasmid

#11464

Depositor

Jon Ashwell

Article

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Insert

mciap2mut (Birc3 Mouse)

Tags

flag

Expression

Mammalian

Mutation

H570A

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-Centrin3

Plasmid

#136806

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-DDK

Plasmid

#137764

Purpose

Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

DDK

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-CjCAS9-pA

Plasmid

#131543

Purpose

Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

Insert

CjCas9

Use

CRISPR

Tags

FLAG-SV40NLS, SV40NLS, and polyA

Expression

Mammalian

Mutation

Human codon-optimized

Promoter

CAG, T3

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcmvtag2Bmciap2

Plasmid

#11463

Depositor

Jon Ashwell

Article

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Insert

mciap2 (Birc3 Mouse)

Tags

flag

Expression

Mammalian

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-GoldenGate-VP64

Plasmid

#47389

Purpose

Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

Type

Empty backbone

Use

TALEN

Tags

FLAG, HA, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-PKI WT

Plasmid

#118300

Purpose

Expression of PKIa

Depositor

Philip Stork

Article

Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

Insert

PKI wt

Tags

Flag and GFP

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ar1

Plasmid

#225219

Purpose

Expression androgen receptor 1 in mammalian cells

Depositor

Deshou Wang

Article

Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

Insert

androgen receptor 1

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Ppst93-ANME2bHR1 MCR-NG

Plasmid

#192774

Purpose

Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ppst93-ANME1G37 MCR-NG

Plasmid

#192773

Purpose

Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pFU-MVIIA-PC

Plasmid

#24148

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-mCherry (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Expression

Mammalian

Available Since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-LacR-TopBP1

Plasmid

#31313

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Tags

FLAG and LacR

Expression

Mammalian

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
TALEN1257

Plasmid

#32280

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting hey2 (hey2 Zebrafish)

Use

T7

Tags

3X FLAG and WT FOKI

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pTC-ApoE-Tet-YAP

Plasmid

#85580

Purpose

Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liver

Depositor

Ursula Ehmer

Article

Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

Inserts

CreER
YAP

Use

Cre/Lox

Tags

Flag (2x)

Expression

Mammalian

Promoter

ApoE.HCR.hAAT and TRE

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-CPZ-TAN3 vpr

Plasmid

#115833

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-TAN3 Vpr (DQ374658)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 hSmad1-GM

Plasmid

#22992

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S210A ,T202A, S191A, S198A

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
1199 pAdTrack FKHR AAA

Plasmid

#9037

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR AAA (FOXO1 Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pOZ-N-FH BRCC36

Plasmid

#27496

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

BRCC36 (BRCC3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only