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pHH0103_RIMBP3-1/3

Plasmid

#91331

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3-1/3 (RIMBP3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCF1-1/2

Plasmid

#91305

Purpose

Protein expression and purification of human SH3 domain construct NCF1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCF1-1/2 (NCF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MPP1-1/1-IS2/2

Plasmid

#91307

Purpose

Protein expression and purification of human SH3 domain construct MPP1-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MPP1-1/1-IS2/2 (MPP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MPP4-1/1-IS2/3

Plasmid

#91308

Purpose

Protein expression and purification of human SH3 domain construct MPP4-1/1-IS2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MPP4-1/1-IS2/3 (MPP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-4/4

Plasmid

#91257

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-4/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MPP4-1/1-IS1/3

Plasmid

#91267

Purpose

Protein expression and purification of human SH3 domain construct MPP4-1/1-IS1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MPP4-1/1-IS1/3 (MPP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3KBP1-3/3

Plasmid

#91250

Purpose

Protein expression and purification of human SH3 domain construct SH3KBP1-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3KBP1-3/3 (SH3KBP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_C_Strep

Plasmid

#101652

Purpose

Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMflCT-o4

Plasmid

#101313

Purpose

Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Inserts

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PatoK-OG241

Plasmid

#72840

Purpose

NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoK

Expression

Bacterial

Promoter

PatoK

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PatoK-LOG241

Plasmid

#72848

Purpose

NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoK

Expression

Bacterial

Promoter

PatoK

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway mmu SE-Zic2 U

Plasmid

#89390

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Zic2 (Zic2 Mouse)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only
E1b-GFP-Tol2-Gateway mmu SE-Zic2 T

Plasmid

#89389

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Zic2 (Zic2 Mouse)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only
E1b-GFP-Tol2-Gateway dre SE-zic2a R

Plasmid

#89387

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-zic2a M

Plasmid

#89382

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-zic2a L

Plasmid

#89381

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only
E1b-GFP-Tol2-Gateway dre SE-zic2a O

Plasmid

#89384

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
E1b-GFP-Tol2-Gateway dre SE-zic2a N

Plasmid

#89383

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0-GL-2-3 perfect copies

Plasmid

#82441

Purpose

Dual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

Luciferase

Mutation

The complementary region for GL2 siRNA control wa…

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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