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pJW2072

Plasmid

#154334

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4RA-P2A-Puro

Plasmid

#112672

Purpose

Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4RA

Use

Lentiviral

Tags

FLAG

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-PHD2

Plasmid

#223552

Purpose

Lentivirus transfer plasmid for expression of full length human PHD2

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3RA-P2A-GFP-PGK-Puro

Plasmid

#110868

Purpose

Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-PGK-Puro

Plasmid

#110860

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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YW249_Pzipt17-loxP-H2BGFP-miniMOS

Plasmid

#196063

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

Depositor

Wenxing Yang

Article

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

H2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR

Expression

Worm

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-P2A-Puro

Plasmid

#110853

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-P2A-Puro

Plasmid

#110851

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1927

Plasmid

#163093

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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