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pJCSUP35

Plasmid

#1088

Depositor

Susan Lindquist

Publication

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

HIS tag

Expression

Bacterial

Available since

May 26, 2005

Availability

Academic Institutions and Nonprofits only
pCAGGS SARS-CoV-2 BA.1 Spike

Plasmid

#185452

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

Depositor

Marceline Côté

Publication

Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

Insert

SARS-CoV-2 Spike BA.1 (S )

Expression

Mammalian

Mutation

Contains the following mutations: A67V, Δ69-70, T…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pSBbi G418 Hu CD19

Plasmid

#183247

Purpose

Expression of human CD19 antigen in a Sleeping Beauty transposon vector

Depositor

Alexander McLellan

Publication

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

CD19 (CD19 Human)

Expression

Mammalian

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry C1 Omp25

Plasmid

#157758

Purpose

Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

Depositor

Marc Tramier

Publication

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

SYNJ2bp (SYNJ2BP Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-eGFP-Z1

Plasmid

#135049

Purpose

Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.

Depositor

Jordan Green

Publication

Yang et al PLoS One. 2026 Jul 23;21(7):e0352468. do

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV Promoter

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAGGS-synPCB2.1

Plasmid

#139478

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Publication

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1

Expression

Mammalian

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-APEX2

Plasmid

#109420

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Publication

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain

Plasmid

#79554

Purpose

Expression of catalytic domain of mouse Tet2

Depositor

Silvia Monticelli

Publication

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

aa990-1912

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
cpVenus-Gγ2

Plasmid

#69626

Purpose

The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

Depositor

Dorus Gadella

Publication

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

cp173Venus-Ggamma2 (GNG2 Synthetic)

Tags

ciruclar permutated Venus (cp173V)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-Slow-FT

Plasmid

#31918

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Slow-FT

Tags

His

Expression

Bacterial

Mutation

M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

Promoter

araB

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-2T FRB

Plasmid

#26607

Depositor

Jie Chen

Publication

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Tags

GST

Expression

Bacterial

Mutation

FRB domain only (aa 2015-2114)

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3-NPY-ClopHensor

Plasmid

#25939

Depositor

Daniele Arosio

Publication

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)

Tags

NPY and Strep-II

Expression

Mammalian

Mutation

Neuropeptide Y localization signal

Available since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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PKM2 (3GQY)

Plasmid

#25360

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PKM2_1 (PKM Human)

Tags

His

Expression

Bacterial

Available since

Aug. 19, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
peCALWY-NB

Plasmid

#22239

Depositor

Maarten Merkx

Publication

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY non-binding variant

Expression

Mammalian

Mutation

C284S, C287S, C413S and C416S mutations linker c…

Available since

Jan. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pTYF-mCMV/SYN-EGFP

Plasmid

#19975

Depositor

Sergey Kasparov

Publication

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMV/SYN-EGFP

Expression

Mammalian

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCR3.1 GSK3beta S9A HA

Plasmid

#15994

Depositor

Morris Birnbaum

Publication

Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

Insert

GSK3beta (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A: constitutively active

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

Plasmid

#193356

Purpose

VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs

Depositor

Hans Schöler

Publication

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise2-Caspase-1-mNeonGreen

Plasmid

#166784

Purpose

FRET analysis (pro caspase-1 cleavage) in living cells

Depositor

Hao Wu

Publication

Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

Insert

mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)

Use

Lentiviral

Tags

mNeonGreen and mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

Plasmid

#126705

Purpose

donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Publication

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

GFP

Use

CRISPR

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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