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pCAGGS_cyt-AKAR5

Plasmid

#114138

Purpose

Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5 with C-terminal nuclear export signal (NES)

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(S11A)-mPAGFP

Plasmid

#114134

Purpose

Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CαN-mPAGFP

Plasmid

#114114

Purpose

Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU11.1-2.2

Plasmid

#115528

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 PUM FL

Plasmid

#112997

Purpose

For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

pumilio (pum Fly)

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 N1(1-259)

Plasmid

#89830

Purpose

Express GATA3 N1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

N1 (AA 1-259)

Promoter

CMV

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 C1(295-443)

Plasmid

#90316

Purpose

Express GATA3 C2 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

GATA3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

C2 (AA 295-443)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 N2(1-294)

Plasmid

#89831

Purpose

Express GATA3 N2 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

N2 (AA 1-294)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-565-YFP-566CT

Plasmid

#41677

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; YFP inserted d…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-570-CFP-571CT

Plasmid

#41680

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; CFP inserted d…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-570-YFP-571CT

Plasmid

#41679

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; YFP inserted d…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-565-CFP-566CT

Plasmid

#41678

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; CFP inserted d…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-mOC-STAMP (N162D)-GFP

Plasmid

#73580

Purpose

Lentivirus expression of mOC-STAMP with N162D mutation

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Lentiviral

Tags

GFP

Mutation

changed asparagin (N) 162 to Aspartic Acid (D)

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3H04

Plasmid

#70971

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf6 (Taf6 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G05

Plasmid

#70809

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIbeta (CkIIbeta Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B07

Plasmid

#70819

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIalpha (CkIIalpha Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-IRES-miR34a-P6

Plasmid

#64790

Purpose

Luciferase expression driven by miR-34a promoter P6

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P6 (MIR34A Human)

Use

Luciferase

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P3

Plasmid

#64787

Purpose

Luciferase expression driven by miR-34a promoter P3

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P3 (MIR34A Human)

Use

Luciferase

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P5

Plasmid

#64789

Purpose

Luciferase expression driven by miR-34a promoter P5

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P5 (MIR34A Human)

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

Plasmid

#65846

Purpose

Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Tags

GFP

Expression

Mammalian

Mutation

R5A-K6A

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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