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Plasmid#124356PurposePlasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxinDepositorInsertgp41-1 N-intein and C-intein (bicistronic)
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationPromoterSTCAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-GDH
Plasmid#108954Purposeheterologous expression of GDH of B. megaterium in E. coliDepositorInsertGlucose dehydrogenase IV (GDH IV)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBHSTC97
Plasmid#124352PurposePlasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxinDepositorInsertNpuDnaBΔ283 mini-intein
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationDeletion of 283-residue endonuclease domainPromoterSTCAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-MVK mazei
Plasmid#108947Purposeheterologous expression of MVK of M. mazei in E. coliDepositorInsertMevalonate kinase (MVK)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-HMGS
Plasmid#108945Purposeheterologous expression of HMGS of E. faecalis in E. coliDepositorInsertHMG-CoA synthase (HMGS)
UseTagsHis6-TagExpressionBacterialMutationA110GPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-MVK jannaschii
Plasmid#108948Purposeheterologous expression of MVK of M. jannaschii in E. coliDepositorInsertMevalonate kinase (MVK)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-IDI2
Plasmid#108951Purposeheterologous expression of IDI2 of T. thermophilus in E. coliDepositorInsertIsopentenyldiphosphat isomerase 2 (IDI2)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-AACT
Plasmid#108944Purposeheterologous expression of AACT of M. sedula in E. coliDepositorInsertAcetoacetyl-CoA synthase (AACT)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
GB1-GyrA
Plasmid#128738PurposeExpresses GB1 fused to GyrA intein at the C-terminalDepositorInsertGB1
UseTagsGyrAExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHBSTC147
Plasmid#124355PurposePlasmid expressing inactive gp41-1 intein inserted into CcdA antitoxinDepositorInsertgp41-1 intein (C1G)
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationC1GPromoterSTCAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBHDuet40
Plasmid#124358PurposeIPTG-inducible vector encoding the N-terminal gp41-1 split fragment with N-terminally His-tagged GB1DepositorInsertgp41-1 N-intein
UseTagsHis6-GB1ExpressionBacterialMutationPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBHBAD42
Plasmid#124359PurposeArabinose-inducible vector encoding the C-terminal gp41-1 split fragment fused to GB1DepositorInsertgp41-1 C-intein
UseTagsGB1 added during cloningExpressionBacterialMutationPromoterParaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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MBP-TEV-Gly-RadA C46
Plasmid#128736PurposeExpresses PhoRadA intein split at residue 46 from the C-terminalDepositorInsertRadA
UseTagsHisx6-MBPExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAS007
Plasmid#164868PurposeThis plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.DepositorInsertphiKO2 operators
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEV-Gly-RadA∆C46
Plasmid#128702PurposeExpresses PhoRadA intein split at residue 126 from the N-terminalDepositorInsertRadA
UseTagsHisx6-MBPExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHBSTC159
Plasmid#124353PurposePlasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxinDepositorInsertNpuDnaBΔ283 (C1G) mini-intein
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationDeletion of 283-residue endonuclease domain, C1GPromoterSTCAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBSTC146
Plasmid#124357PurposePlasmid expressing inactive N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxinDepositorInsertgp41-1 N-intein (C1G) and C-intein (bicistronic)
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationC1GPromoterSTCAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNO286-3
Plasmid#107746PurposeKey genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)DepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationDeleted AA 223 and 512PromoterJ23106 and J23108Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-SIN6.1 CBLucWm1A
Plasmid#44163DepositorInsertfirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterCB (CMV enhancer/chicken beta-actin promoter)Available sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only