We narrowed to 32,439 results for: Eng;

1. pZ_lacO-cP-AOX1-eGFP

   Plasmid

   #126720

   Purpose

   yeast plasmid overexpressing eGFP

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   egfp
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p1585588

   Plasmid

   #62620

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   dihydroflavonol 4-reductase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   leucoanthocyanidin reductase
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCHC025 (∆CBBp)

   Plasmid

   #226203

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
   

   Use

   Synthetic Biology

   Mutation

   ∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpAp

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCHC024 (∆SH)

   Plasmid

   #226202

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for soluble hydrogenase operon
   

   Use

   Synthetic Biology

   Mutation

   ∆hoxFUYHWI ∆hypA2B2F2

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCHC023 (∆MBH)

   Plasmid

   #226201

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for membrane bound hydrogenase operon
   

   Use

   Synthetic Biology

   Mutation

   ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCHC027 (∆pemK)

   Plasmid

   #226204

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Upstream/downstream homology arms for addiction system toxinPemK.
   

   Use

   Synthetic Biology

   Available Since

   Nov. 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pACB108

   Plasmid

   #222207

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACB109

   Plasmid

   #222208

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (K10N)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Lys10 to Asn

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACB110

   Plasmid

   #222209

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (G258D)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Gly258 to Aspartate

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. (R)pGPD1(D)_P1(SBE109)

    Plasmid

    #194993

    Purpose

    promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (R) pGPD (D)
    

    Expression

    Bacterial

    Available Since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. WT_IFL_A28C_R

    Plasmid

    #200184

    Purpose

    WT PA14 I-F Leader and A28C Repeat

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Wildtype I-F Leader and a mutant I-F Locus (A28C)
    

    Expression

    Bacterial

    Mutation

    A28C in the I-F repeat

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. (F)pGPD(G)_P2 (SBE110)

    Plasmid

    #194992

    Purpose

    promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (F) pGPD (G)
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. (I) pADH2 (J) P3 (SBE114)

    Plasmid

    #187629

    Purpose

    promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (I) pADH2 (J)
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. (F) pADH2 (G)_P2 (SBE113)

    Plasmid

    #187628

    Purpose

    promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (F) pADH2 (G)
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

    Plasmid

    #195054

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pLDP1

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

    Plasmid

    #195053

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pFBA

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

    Plasmid

    #195052

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pXYL

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

    Plasmid

    #195051

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pGPD

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

    Plasmid

    #195050

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pADH2

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. (F) pXYL (G) P1 (SBE216)

    Plasmid

    #195017

    Purpose

    promoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (F) pXYL (G)
    

    Expression

    Bacterial

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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