We narrowed to 2,247 results for: eda

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L1-Ck1-hs1-l-chlo

Plasmid

#163937

Purpose

For integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha trnG_trnfM region left homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1-Ck4-hs1-r-chlo

Plasmid

#163940

Purpose

For integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha trnG_trnfM region right homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1-Ck4-hs2-r-chlo

Plasmid

#163941

Purpose

For integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha rbcL_trnR region right homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_MpU6

Plasmid

#136120

Purpose

Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpU6

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS_Cas9-NLS

Plasmid

#136121

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_AtcoCas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS12-MpER-Targ

Plasmid

#136094

Purpose

CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

ER targeting peptide from predicted chitinase Mapoly0069s0092

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_eGFP-GHtag-HDEL

Plasmid

#136096

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Plasmid

#167478

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Plasmid

#167479

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisFlag-rUSP19ER-CAmut

Plasmid

#155237

Purpose

for mammalian overexpression of the mutated ER isoform of rat USP19

Depositor

Simon Wing

Article

Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

Insert

USP19 (ER isoform) (Usp19 Rat)

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Cys(545) to Ala

Promoter

CMV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPV-1.8k-Venus-WPRE

Plasmid

#156398

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSP-0.8k-Venus-WPRE

Plasmid

#156397

Purpose

Expresses Venus under SP gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSP-1.7k-Venus-WPRE

Plasmid

#156396

Purpose

Expresses Venus under SP gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSERT-0.5k-Venus-WPRE

Plasmid

#156395

Purpose

Expresses Venus under SERT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfvAChT-1.1k-Venus-WPRE

Plasmid

#156390

Purpose

Expresses Venus under vAChT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfCCK-3.9k-Venus-WPRE

Plasmid

#156391

Purpose

Expresses Venus under CCK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pKI-cjPLP1e2-A39T

Plasmid

#129752

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

PLP1 (PLP1 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

A39T

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjPLP1e2-P15L

Plasmid

#129753

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

PLP1 (PLP1 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

P15L

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-S15

Plasmid

#127821

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-T136D-S15

Plasmid

#127824

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

T136D

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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