We narrowed to 1,217 results for: poli

1. pET14b-dabAB2 C351A

   Plasmid

   #133014

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Use

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Mutation

   C351A

   Promoter

   t7

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCRII apoA-Ib

   Plasmid

   #121896

   Purpose

   Plasmid for making zebrafish apoA-Ib in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoA1b (apoa1b Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCRII apoBb.2

   Plasmid

   #121899

   Purpose

   Plasmid for making zebrafish apoBb.2 in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoBb.2 (apobb.2 Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCRII apoA-IVb.3

   Plasmid

   #121905

   Purpose

   Plasmid for making zebrafish apoA-IVb.3 in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   apoa4b.3 (apoa4b.3 Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCRII apoA-IVa

   Plasmid

   #121902

   Purpose

   Plasmid for making zebrafish apoA-IVa in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoA4a (apoa4a Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PCRII apoA-Ia

   Plasmid

   #121895

   Purpose

   Plasmid for making zebrafish apoA-Ia in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   apoA-Ia (apoa1a Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDECKO-mCherry MALAT1_Enhancer.4

   Plasmid

   #78539

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDECKO-mCherry MALAT1_Enhancer.3

   Plasmid

   #78538

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDECKO-mCherry MALAT1_Exon.3

   Plasmid

   #78542

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDECKO-mCherry MALAT1_Enhancer.2

    Plasmid

    #78537

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 AKT1

    Plasmid

    #67102

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCellFree_G03 BMPR1A

    Plasmid

    #67105

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BMPR1A (BMPR1A Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCellFree_G03 EHD2

    Plasmid

    #67107

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    EHD2 (EHD2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCellFree_G03 GTF2H1

    Plasmid

    #67108

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H1 (GTF2H1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

    Plasmid

    #89888

    Purpose

    Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    mCherry-KRasG12V
    

    Use

    Unspecified

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMHE-mH33B-mClover3

    Plasmid

    #171496

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H3.3B (H3f3b Mouse)
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Use

    Tags

    Cerulean

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRMC2-sec:msfGFP

    Plasmid

    #194914

    Purpose

    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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