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pET51b(+)_MBP-nLucCP-65/66

Plasmid

#113920

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/66

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-LUCIDRS-MTX

Plasmid

#113925

Purpose

Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

LUCID_RS-MTX

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

Plasmid

#104619

Purpose

Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

Depositor

Tudor Fulga

Publication

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10

Tags

FLAG

Expression

Insect

Promoter

hsp70

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pG-HIV-SIN-2LTR

Plasmid

#104591

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Publication

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of self-inactivating HIV-based vectors

Use

Cloning vector

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 5kb promoter/AcGFP

Plasmid

#98101

Purpose

Zebrafish aBb crystallin 5kb promoter segment driving GFP expression

Depositor

Mason Posner

Publication

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 5 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 5 kb promoter fragment …

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 4kb promoter/AcGFP

Plasmid

#98100

Purpose

Zebrafish aBb crystallin 4kb promoter segment driving GFP expression

Depositor

Mason Posner

Publication

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 4 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 4 kb promoter fragment …

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SPA mimic WT

Plasmid

#92350

Purpose

To mimic SPA RNA processing and expression

Depositor

Ling-Ling Chen

Publication

Wu et al Mol Cell. 2016 Nov 3;64(3):534-548. doi:

Insert

SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLX302 PCSK5 (T288P)-V5 puro

Plasmid

#98709

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMT2 Cav2.2 DomI and DomII

Plasmid

#89891

Purpose

Mammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2

Depositor

Annette Dolphin

Publication

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

Cacna1b (CACNA1B )

Expression

Mammalian

Mutation

Stop codon after amino acid 1154 (this is a trunc…

Promoter

adenovirus major late promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1702

Plasmid

#78195

Purpose

optogenetic activation of IL2 neuron in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

ChR2(gf)

Expression

Worm

Promoter

lag-2

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pJL1603

Plasmid

#78193

Purpose

Ce cha-1 expression in SMB, SIA neurons in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

odr-2

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1602

Plasmid

#78192

Purpose

Ce cha-1 expression in SIA, RMD neurons in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

trp-1

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1601

Plasmid

#78191

Purpose

Ce cha-1 expression in IL2, SIA neurons in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

osm-6

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
FCIV1-GR SS287AA

Plasmid

#72703

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Publication

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

SS287AA

Promoter

ubiquitin

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pRS425-M25-Afg1

Plasmid

#61720

Purpose

yeast Afg1 AAA protein in yeast expression vector

Depositor

Oleh Khalimonchuk

Publication

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

Afg1 (AFG1 Budding Yeast)

Expression

Yeast

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only
pAN-PA5-RFP

Plasmid

#62251

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Publication

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA5

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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SNV-memTdTomato

Plasmid

#53123

Purpose

retroviral vector expressing membrane TdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

Depositor

Jeanette Hyer

Publication

Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

Insert

memTdTomato

Use

Retroviral; Expression in chicken cells

Promoter

SNV (spleen necrosis virus)

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
SNV-lacZ

Plasmid

#53125

Purpose

retroviral vector expressing lacZ, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

Depositor

Jeanette Hyer

Publication

Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

Insert

lacZ (lacZ E.coli)

Use

Retroviral; Expression in chicken cells

Promoter

SNV (spleen necrosis virus)

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MsMUT_sgRNA1

Plasmid

#111296

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Publication

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine MUT

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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MsMUT_sgRNA3

Plasmid

#111297

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Publication

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA3 against murine MUT

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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