We narrowed to 15,989 results for: LEA

1. pLenti-L3US2-RFP-HDAC3

   Plasmid

   #83966

   Purpose

   Lentiviral CRISPR HDAC3 dual gRNA targeting vector

   Depositor

   Eleanor Chen

   Insert

   HDAC3 dual gRNAs (HDAC3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBHSTC97

   Plasmid

   #124352

   Purpose

   Plasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin

   Depositor

   Hideo Iwai

   Article

   Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

   Insert

   NpuDnaBΔ283 mini-intein
   

   Tags

   CcdA-C (SFAD)

   Expression

   Bacterial

   Mutation

   Deletion of 283-residue endonuclease domain

   Promoter

   STC

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pREXC3H

   Plasmid

   #47078

   Purpose

   FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site and 10x His tags

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Lactococcus lactis expression

   Tags

   3C protease site (PreScission site) and decaHis (…

   Promoter

   NIS (nisin)

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS_2paLINX

   Plasmid

   #129655

   Purpose

   2P-activatable light-inducible nuclear exporter

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paLINX
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046409

   Plasmid

   #153939

   Purpose

   Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGBW-m4046479

   Plasmid

   #145495

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAGGS-Trex2

   Plasmid

   #79246

   Purpose

   Expression vector for Trex2, which is a non-processive 3' exonuclease.

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

   Insert

   Trex2 (Trex2 Mouse)
   

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. CBX2

   Plasmid

   #25158

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBX2_04 (CBX2 Human)
   

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. GALTA-c001

   Plasmid

   #79645

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GALT (GALT Human)
   

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4046323

    Plasmid

    #149100

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pEG408

    Plasmid

    #40086

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (aa 345 - 468) (mex-5 Nematode)
    

    Tags

    Dendra2 PFAF, S-peptide, and TEV protease cleavag…

    Expression

    Worm

    Mutation

    contains aa 345 - 468

    Promoter

    pie-1

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pGBW-m4134165

    Plasmid

    #152085

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMAH-Golgi-bapaUGAc

    Plasmid

    #201675

    Purpose

    boronic acid- and peptide-assisted UDP-GlcNAc sensor in Golgi

    Depositor

    Huiwang Ai

    Article

    Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

    Insert

    Golgi-bapaUGAc
    

    Tags

    Golgi leading sequence

    Expression

    Mammalian

    Promoter

    CMV SP6

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4258306

    Plasmid

    #154795

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;V5

    Expression

    Mammalian

    Mutation

    H. sapiens recode 1

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGBW-m4046348

    Plasmid

    #149108

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pBXNH3A

    Plasmid

    #47075

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His, 3C protease cleavage site and Avi-tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), Avi-tag, and…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPTM p62 PB1 ZZ (1-167)

    Plasmid

    #210429

    Purpose

    Bacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage site

    Depositor

    Carsten Sachse

    Article

    Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

    Insert

    p62 PB1 ZZ (1-167) (NUP62 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJA50

    Plasmid

    #59931

    Purpose

    gRNA for cleavage at unc-58(e665) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    unc-58(e665) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBXNAC3GH

    Plasmid

    #47073

    Purpose

    FX cloning E.coli expression vector with araBAD promoter, N-terminal Avi-tag, C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), Avi-tag, GFP…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4133326

    Plasmid

    #152113

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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