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pET21a - TryX

Plasmid

#89623

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Tryparedoxin

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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Stagia3-Cre

Plasmid

#73991

Purpose

Cre in Stagia3 backbone; vector-Cre

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Cre

Tags

Myc

Expression

Mammalian

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1942_ hU6-PV2-lox2272-BbsI-ENTRY-gRNA

Plasmid

#89055

Purpose

PV2 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1720_hU6-PV1-FRT-lox2272-BbsI-ENTRY-gRNA

Plasmid

#89053

Purpose

PV1 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpA

Plasmid

#87576

Purpose

PV3 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iRFP720

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpA

Plasmid

#87582

Purpose

PV3 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW759_pCAG-PV2-lox2272-GFP-BGHpA

Plasmid

#87575

Purpose

PV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA

Plasmid

#87580

Purpose

PV1 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

Neomycin Resistance Gene

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW807_pCAG-PV4-loxP-mCherry-BGHpA

Plasmid

#87583

Purpose

PV4 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW770_pCAG-PV4-loxP-mRuby2-BGHpA

Plasmid

#87577

Purpose

PV4 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW707_pCAG-PV3-FRT-loxP-mCherry

Plasmid

#87571

Purpose

PV3 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBW705_pCAG-PV1-FRT-lox2272-STOP

Plasmid

#87569

Purpose

PV1 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

Neomycin Resistance Gene

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBW706_pCAG-PV2-lox2272-GFP

Plasmid

#87570

Purpose

PV2 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-EGFP-NLS

Plasmid

#87778

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Tags

3xFLAG and NLS

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

Plasmid

#87777

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7-EE

Tags

3xFLAG and NLS

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*-(ΔLF)

Plasmid

#80045

Purpose

Expression of Vpr binding mutant EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Mutation

Vpr binding site LF45 in p6 mutated

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔI)

Plasmid

#80048

Purpose

Expression of EPN-01 assembly mutant L33R

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(L33R)

Tags

Myc

Expression

Mammalian

Mutation

L33R

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL1+L2)

Plasmid

#80051

Purpose

Expression of EPN-01 mutated in both L domains

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP/deltaYP)

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA, YP37AA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔI)

Plasmid

#80041

Purpose

Expression of EPN-01 assembly mutant L33R

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Mutation

changed Leu33 in I3-01 to Ala

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-cTEV-PuroR

Plasmid

#80958

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

c-terminal split TEV

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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