We narrowed to 19,007 results for: LEA

1. p3E-2a-tdTomato

   Plasmid

   #67707

   Purpose

   3' entry 2A-tdTomato

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   2A-tdTomato
   

   Use

   Tags

   Expression

   Mutation

   G172S (please see depositor's comments below)

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-AKAR4

   Plasmid

   #61619

   Purpose

   FRET-based biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   AKAR4
   

   Use

   Tags

   Cerulean and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 6972 pPROEX-HTb-SSA1

   Plasmid

   #1231

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   Ssa1 (SSA1 Budding Yeast)
   

   Use

   Tags

   his tag (tev cleavable)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 9, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pMSCV-Peredox-mCherry-NLS

   Plasmid

   #32385

   Depositor

   Gary Yellen

   Article

   Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

   Insert

   Peredox-mCherry-NLS
   

   Use

   Retroviral

   Tags

   Nuclear Localization Seq

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOPINVL

   Plasmid

   #26040

   Depositor

   Ray Owens

   Article

   Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

   Insert

   u-LacZ
   

   Use

   Tags

   u-phosphatase secretion leader

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPINVH

   Plasmid

   #26041

   Depositor

   Ray Owens

   Article

   Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

   Insert

   u-LacZ-KHHHHHH
   

   Use

   Tags

   KHHHHHH and u-phosphatase secretion leader

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EBFP2-PCNA-19-SV40NLS-4

   Plasmid

   #55250

   Purpose

   Localization: Nucleus, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   3xSV40 NLS and EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pYEXNH3

   Plasmid

   #49024

   Purpose

   FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 6975 pPROEX-HTb-SIS1

   Plasmid

   #1233

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   sis1 (SIS1 Budding Yeast)
   

   Use

   Tags

   his tag (tev cleavable)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

10. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69546

    Purpose

    Tet-On system expressing MyoD-2A-GFP-2xNLS.

    Depositor

    Charles Gersbach

    Inserts

    eGFP
    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Expression

    Mutation

    Removed stop codon to fuse it to the 2A-eGFPnls i…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEGFP-PCNA-19-SV40NLS-4

    Plasmid

    #56469

    Purpose

    Localization: Nucleus, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pcDNA_DiCre2_60.F2

    Plasmid

    #60210

    Purpose

    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)

    Depositor

    Jean-Paul Herman

    Insert

    Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)
    

    Use

    Tags

    FKBP12(F36V) and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    FKBP12 has the F36V mutation; amino acids 60-343 …

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBXC3GH

    Plasmid

    #47070

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 6974 pPROEX-HTb-SSB1

    Plasmid

    #1232

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    ssb1 (SSB1 Budding Yeast)
    

    Use

    Tags

    his tag (tev cleavable)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 9, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pICXNH3

    Plasmid

    #49020

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCURE

    Plasmid

    #23013

    Depositor

    James Bowie

    Article

    Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

    Insert

    I-CreI homing endonuclease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I-CreI expression is controlled by the arabinose …

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

17. p3E-2a-6MTnlstdTomato

    Plasmid

    #67699

    Purpose

    3' entry clone with 6xmyc nuclear localized tdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    tdTomato
    

    Use

    Tags

    2A-6xMyc-NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mTagRFP-PCNA-19-SV40NLS-4

    Plasmid

    #57994

    Purpose

    Localization: Nucleus, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pMtB

    Plasmid

    #32096

    Depositor

    Christopher Buck

    Article

    Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

    Insert

    MCV Small t antigen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aberrant splicing leads to additional protein pro…

    Promoter

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA_DiCre2_59.F2

    Plasmid

    #60209

    Purpose

    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)

    Depositor

    Jean-Paul Herman

    Insert

    Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)
    

    Use

    Tags

    FKBP12(F36V) and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    FKBP12 has the F36V mutation; amino acids 19-59 o…

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge