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Plasmid#156166PurposeLentiviral vector for expression of RAP1A-WT, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationwild type formPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)
Plasmid#185706PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLSDepositorInsertCas9
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-E63-puro
Plasmid#156167PurposeLentiviral vector for expression of RAP1A-E63, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationchanged Glutamine 63 to Glutamic acidPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-V12-puro
Plasmid#156168PurposeLentiviral vector for expression of flag-RAP1B-V12, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-PH-BTKx1
Plasmid#183651PurposePtdIns(3,4,5)P3 biosensorDepositorInsertBTK (BTK Human, Frog)
UseTagsX. laevis map2k1.L(32-44)-EGFPExpressionMammalianMutationAmino acids 2-170PromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)
Plasmid#185701PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(FCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)
Plasmid#185705PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(PDCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)
Plasmid#185703PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(SCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)
Plasmid#185704PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(PCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)
Plasmid#185702PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(BCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-PH-BTKx1
Plasmid#183653PurposePtdIns(3,4,5)P3 biosensorDepositorInsertBTK (BTK Human, Frog)
UseTagsX. laevis map2k1.L(32-44)-mCherryExpressionMammalianMutationAmino acids 2-170PromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-N17-puro
Plasmid#156169PurposeLentiviral vector for expression of flag-RAP1B-N17, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Serine 17 to AsparaginePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-WT-puro
Plasmid#156170PurposeLentiviral vector for expression of RAP2A-WT, with puromycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsExpressionMammalianMutationwild type formPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-V12-puro
Plasmid#156171PurposeLentiviral vector for expression of RAP2A-V12, with puromycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-N17-puro
Plasmid#156172PurposeLentiviral vector for expression of RAP2A-N17, with puromycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsnoneExpressionMammalianMutationchanged Serine 17 to AsparaginePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV HA TrkB-T1
Plasmid#39980DepositorInsertTrkB.T1 (Ntrk2 Rat)
UseTagsBM40 signal sequence and HAExpressionMammalianMutationTrkB.T1 variant; endogenous signal sequence was r…PromoterCMVAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-Rab11a
Plasmid#72902PurposeRecycling endosome-targeted mCherry-tagged recruiter constructDepositorInsertRab11a (RAB11A Human)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationfragment a.a. 2-216PromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Eef25UTR-renilla
Plasmid#38235DepositorInsertEef2 5'UTR / Renilla (Eef2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationEef2 5' UTR fused to renilla ORFPromoterEndogenous Eef2Available sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-Rab11-Blasticidin
Plasmid#120717PurposeExpresses recycling endosome-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertRab11a
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationAmino acids 2-216PromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-Rab5-Blasticidin
Plasmid#120715PurposeExpresses early endosome-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertRab5a
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationAmino acids 2-215PromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only