We narrowed to 1,255 results for: cfp

1. pCEE

   Plasmid

   #159746

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

   Plasmid

   #114079

   Purpose

   CAG promoter expression plasmid for human codon optimized AsBE1.1

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cer(1-158)-gamma-1 in pcDNAI/Amp

   Plasmid

   #55623

   Purpose

   An amino-terminal mCerulean fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   Cer(1-158)-gamma-1 (GNGT1 Aequorea victoria, Bovine)
   

   Tags

   Cer(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-1 was amplified by PCR, which added a BamH…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cer(1-158)-gamma-2 in pcDNAI/Amp

   Plasmid

   #55624

   Purpose

   An amino-terminal mCerulean fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   Cer(1-158)-gamma-2 (Gng2 Aequorea victoria, Mouse)
   

   Tags

   Cer(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-2 was amplified by PCR, which added a BamH…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cer(1-158)-gamma-7 in pcDNAI/Amp

   Plasmid

   #55626

   Purpose

   An amino-terminal mCerulean fragment was fused to Ggamma-7. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   Cer(1-158)-gamma-7 (GNG7 Human, Aequorea victoria)
   

   Tags

   Cer(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-7 was amplified by PCR, which added a BamH…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cer(1-158)-gamma-10 in pcDNAI/Amp

   Plasmid

   #55627

   Purpose

   An N-terminal mCerulean fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   Cer(1-158)-gamma-10 (GNG10 Human, Aequorea victoria)
   

   Tags

   Cer(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-10 was amplified by PCR, which added a Bam…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cer(1-158)-gamma-11 in pcDNAI/Amp

   Plasmid

   #55628

   Purpose

   An N-terminal mCerulean fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   Cer(1-158)-gamma-11 (GNG11 Aequorea victoria, Bovine)
   

   Tags

   Cer(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-11 was amplified by PCR, which added a Bam…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cer(1-158)-gamma-12 in pcDNAI/Amp

   Plasmid

   #55629

   Purpose

   An N-terminal mCerulean fragment was fused to Ggamma-12. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   Cer(1-158)-gamma-12 (GNG12 Human, Aequorea victoria)
   

   Tags

   Cer(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-12 was amplified by PCR, which added a Bam…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-SLC25A40_STOP

   Plasmid

   #161160

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A40 (SLC25A40 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3-Lyso-InPTapp

    Plasmid

    #236000

    Purpose

    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Lyso-InPTapp (PLEKHA1, LAMP1 Human, Synthetic)
    

    Tags

    Citrine, Full-length LAMP1, and truncECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ICUE4

    Plasmid

    #181846

    Purpose

    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    ICUE4 (RAPGEF3 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-NES-ZapCV2

    Plasmid

    #222950

    Purpose

    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2 (NES Synthetic)
    

    Tags

    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-RIα-ICUE4

    Plasmid

    #181848

    Purpose

    ICUE4 cAMP sensor tethered to the PKA RIα subunit.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
    

    Tags

    ECFP, PKA RIα, and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-PDE4D2cat-ICUE4

    Plasmid

    #181847

    Purpose

    ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
    

    Tags

    ECFP, PDE4D2(86-418), and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HL 1745

    Bacterial Strain

    #61160

    Purpose

    Lim Lab Strain

    Depositor

    Han Lim

    Article

    Block et al Nucleic Acids Res. 2012 Oct;40(18):8979-

    Bacterial Resistance

    None

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid

    #107942

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R, and N282A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid

    #114084

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid

    #114082

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

    Plasmid

    #114081

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge