We narrowed to 5,317 results for: golden gate
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Plasmid#120936PurposeMoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertJ23151
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_2_XcmI-LacZ
Plasmid#49297PurposeGolden Gate entry vector at position 2, LacZDepositorTypeEmpty backboneUseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_2_+nls-eGFP-HA-_+1_BK+
Plasmid#49312PurposeGolden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codonDepositorInsertnls-EGFP-HA
UseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E_GGWDest+
Plasmid#49319PurposeDestination vector for Golden Gate assembly; 5' entry vector for Gateway cloningDepositorTypeEmpty backboneUseGateway CloningAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
C90m
Plasmid#120998PurposeMoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.DepositorInsertNahRsfGFP fusion
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C73m
Plasmid#120985PurposeMoClo golden gate assembly CD part for LasR-LSTPLVMI (Adam Meyer's improved LasR activator, selected for dynamic range and low cross-sensitivity with methods from https://doi.org/10.1101/285866).DepositorInsertLasR Adam Meyer
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C62m
Plasmid#120978PurposeMoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.DepositorInsertTraR(W) from Scott and Hasty 2016
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C22m
Plasmid#120963PurposeMoClo golden gate assembly CD part for mSpinach (fluorescent RNA aptamer for DFHBI, suitable for in vivo use; see DOI: 10.1021/sb400089c). Please see Supplemental Documents for annotated Genbank file.DepositorInsertmSpinach
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P12m
Plasmid#120938PurposeMoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pLasTetO
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P15m
Plasmid#120939PurposeMoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinTetO
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P16m
Plasmid#120940PurposeMoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinLacO m
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGDestSC-ATG
Plasmid#49322PurposeDestination vector for Golden Gate assembly; Few restriction sites for subcloning.DepositorTypeEmpty backboneUseGateway CloningAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGEV_3_Linker
Plasmid#49283PurposeGolden Gate entry vector at position 3, small linker insertDepositorTypeEmpty backboneUseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTwist-Kan-HC-L0-TaMOFB1(int)
Plasmid#242005PurposeL0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum MORE FLORET 1 (MOF-1) / MULTI-FLORET SPIKELET 2 (MFS-2) B-subgenome homoeolog (TraesCS2B02G420900)DepositorInsertTaMOF-B1_with_introns
UseSynthetic BiologyExpressionPlantAvailable sinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
T7m
Plasmid#121030PurposeMoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertS. pyogenes tracrRNA terminator
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C108m
Plasmid#121010PurposeMoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized with pdt3 tag
UseSynthetic BiologyTagspdt3 mfLon degradation tagAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C103m
Plasmid#121007PurposeMoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C70m
Plasmid#120982PurposeMoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).DepositorInsertRpaR Adam Meyer
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P61m
Plasmid#120953PurposeMoClo golden gate assembly AB part for pTac (Adam Meyer's improved pTac promoter selected for dynamic range and low cross-sensitivity; unpublished, using strategy from https://doi.org/10.1101/285866).DepositorInsertTac promoter Adam Meyer
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EMMA toolkit
Plasmid kit#1000000119PurposeCollection of Golden Gate vectors and standardized genetic parts for the construction mammalian expression plasmids.DepositorApplicationCloning and Synthetic BiologyVector typeMammalian ExpressionCloning typeGolden GateAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only