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pET SUMO-syt1 (96-421)

Plasmid

#213619

Purpose

Bacterial expression of SUMO-tagged rat syt1 (96-421)

Depositor

Edwin Chapman

Publication

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 (96-421) (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-bII spectrin (repeats 13-15)

Plasmid

#213729

Purpose

Mammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Publication

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bII spectrin (repeats 13-15) (Spnb3 Mouse)

Expression

Mammalian

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR/eCas9-GPIBY55

Plasmid

#213709

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

Depositor

Qilai Huang

Publication

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

CRISPR

Tags

3XFLAG and Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk2b

Plasmid

#208984

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Publication

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk2b (kcnk2b Zebrafish)

Use

Cloning

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA

Plasmid

#201964

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Publication

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E162A,M164A,R167A

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

Plasmid

#201967

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Publication

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R232A, K233A, R234A, K235A, R236A

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

Plasmid

#201968

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Publication

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

W255A,N262E,K266E

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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(B) G418 marker (R) (SBE 137)

Plasmid

#195011

Purpose

geneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+G418+tNOS

Expression

Bacterial

Promoter

pXYL

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pETSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209011

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Publication

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis and msfGFP

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(143-421)msGFP

Plasmid

#209015

Purpose

Overexpression of truncated syt1 tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Publication

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 143-421 only

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B1(E234Q)-HA

Plasmid

#209241

Purpose

Mammalian expression of ATP8B1 mutant E234Q

Depositor

Hye-Won Shin

Publication

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B1 (ATP8B1 Human)

Tags

HA

Expression

Mammalian

Mutation

E234Q

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-Ctdnep1_C-ter

Plasmid

#196525

Purpose

Expression of Ctdnep1_C-ter

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-C-ter (CTDNEP1 Human)

Tags

His

Expression

Bacterial

Mutation

No transmembrane domain

Promoter

T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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p2129

Plasmid

#198143

Purpose

Epitope tagging of effector protein SseJ

Depositor

Michael Hensel

Publication

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PsseJ sseJ::M45 (sseJ Salmonella enterica serovar Typhimurium)

Tags

M45

Expression

Bacterial

Mutation

P290S mutation in sseJ

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLNHA-C1-HsNot2-del409-432_T

Plasmid

#147625

Purpose

Mammalian Expression of DmNot2-del409-432

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del409-432 (Rga Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot3-del607-634_T

Plasmid

#147626

Purpose

Mammalian Expression of HsNot3-del607-634

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-del607-634 (CNOT3 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot3-del632-654_T

Plasmid

#147627

Purpose

Mammalian Expression of HsNot3-del632-654

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3-del632-654 (CNOT3 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2-del344-380_T

Plasmid

#147623

Purpose

Mammalian Expression of DmNot2-del344-380

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del344-380 (Rga Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2-del380-404_T

Plasmid

#147624

Purpose

Mammalian Expression of DmNot2-del380-404

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del380-404 (Rga Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-EGFP-C1-HsNot1iso1_1595-2376_S

Plasmid

#147491

Purpose

Mammalian Expression of HsNot1iso1_1595-2376

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso1_1595-2376 (CNOT1 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del380-404-W507E_S

Plasmid

#147499

Purpose

Mammalian Expression of HsNot2-del380-404-W507E

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-del380-404-W507E (CNOT2 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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