We narrowed to 17,894 results for: emb
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pGEX-4T-3-cytXlATL2
Plasmid#124063PurposeFragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.DepositorInsertcytXIATL2
ExpressionBacterialPromotertacAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP
Plasmid#124066PurposeE. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.DepositorInsertcytATL-R232Q-sfGFP
TagssfGFPExpressionBacterialMutationR232QPromotertrcAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBAC1-CoREST-C
Plasmid#109159PurposeEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
Tags3xFLAGExpressionInsectPromoterPolyhedrinAvailable SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5-LAP-BUB1
Plasmid#115903PurposeUsed for expression of siRNA-resistant BUB1-WT from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFL I3
Plasmid#113514PurposeExpression of I3 in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-STOP-HEC1
Plasmid#114026PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.DepositorInserteGFP-STOP-HEC1 (NDC80 Human)
ExpressionMammalianMutationStart codon of Hec1 is mutated to stop codonAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-biotin-myc- mCE
Plasmid#82475PurposeExpresses mRNA capping enzyme containing N terminal biotin and myc tagDepositorAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 BICC FL
Plasmid#112998PurposeFor protein expression and purification of full-length Drosophila BicCDepositorInsertfull length BicC (BicC Fly)
ExpressionBacterialAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1-HLx6
Plasmid#108135PurposeRecruitable SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]6:SACM1L(521-587) (SACM1L Human)
ExpressionMammalianMutationSACM1L(1-520):[EAAAR]6:SACM1L(521-587)PromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1-HLx4
Plasmid#108134PurposeRecruitable SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]4:SACM1L(521-587) (SACM1L Human)
ExpressionMammalianMutationSACM1L(1-520):[EAAAR]4:SACM1L(521-587)PromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5081
Plasmid#85107PurposePtrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.DepositorInsertYFP-KaiC
TagsYFPExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-RP2-C86Y,P95L
Plasmid#86074PurposeA bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.DepositorInsertRP2 (RP2 Human)
TagsGSTExpressionBacterialMutationChanged cysteine-86 and proline-95 to tyrosine an…PromotertacAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-cystin-CTS
Plasmid#86066PurposeA bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.DepositorInsertCystin (Cys1 Mouse)
TagsGSTExpressionBacterialMutationcontains the region from 28-35PromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD3cC
Plasmid#86972PurposeExpresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)-Halo-PS
Plasmid#86632PurposeExpresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)DepositorInsertAkt2(K14A/R25E)-Halo-PS (AKT2 )
ExpressionMammalianAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(WT)
Plasmid#86576PurposeExpresses cleavable human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
Tags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130Available SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only
