We narrowed to 11,122 results for: HA

1. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60227

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CaMKII-ChrimsonR-tdTomato

   Plasmid

   #99231

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CaMKII

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Kv1.4 K+ channel (extracellular) scFv [L71/5]

   Plasmid

   #190517

   Purpose

   Mammalian Expression of Kv1.4 K+ channel (extracellular) scFV. Derived from hybridoma L71/5.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant scFV (Kcna4 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Kv1.2 K+ channel scFv [K14/16]

   Plasmid

   #182064

   Purpose

   Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kv2.1 K+ channel scFv [K39/25]

   Plasmid

   #182067

   Purpose

   Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K39/25.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GABA(A)R, Gamma2L/S scFv [N452/73]

   Plasmid

   #190555

   Purpose

   Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. DBDmut-CA-RIT-NFAT1

   Plasmid

   #63669

   Purpose

   Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding

   Depositor

   Anjana Rao

   Article

   Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

   Insert

   NFAT1 (Nfatc2 Mouse)
   

   Use

   Retroviral

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pIG-829_HA-GD2-28z_CAR_JUN_Retroviral

   Plasmid

   #207506

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   JUN, HA-GD2-28z_CAR (JUN Human)
   

   Use

   Retroviral

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. iChr2-Notch Mosaic (SO250)

   Plasmid

   #99752

   Purpose

   Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins and Notch signalling genes

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   HIs-H2B-Cherry, H2B-EGFP-V5, HA-H2B-Cerulean, DN-Maml1, NICD-PEST
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid

    #126479

    Purpose

    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Inserts

    Or56a (Or56a Fly)
    Orco (Orco Fly)
    

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens and codon optim…

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. NPY/Neuropeptide Y scFv [L115/13]

    Plasmid

    #190505

    Purpose

    Mammalian Expression of NPY/Neuropeptide Y scFV. Derived from hybridoma L115/13.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    NPY/Neuropeptide Y (Homo sapiens) recombinant scFV (NPY Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN DHFR-HiBiT / Lyt2

    Plasmid

    #179731

    Purpose

    Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites

    Depositor

    James Inglese

    Article

    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

    Insert

    human DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
    

    Use

    Retroviral

    Tags

    HiBiT

    Promoter

    LTR/MMLV

    Available Since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Kir6.1 K+ channel scFv [N366/60]

    Plasmid

    #190547

    Purpose

    Mammalian Expression of Kir6.1 K+ channel scFV. Derived from hybridoma N366/60.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Kir6.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj8 Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)

    Plasmid

    #114095

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RR (E174R/S542R/K607R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R, S542R and K607R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #128589

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    Syn

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX307 hDVL1

    Plasmid

    #102863

    Purpose

    Expresses human DVL1 in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    human Dishevelled 1 (DVL1) (DVL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed alanine 2 to glycine. Likely due to poly…

    Promoter

    EF1A

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-i53-DM

    Plasmid

    #92171

    Purpose

    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    i53-DM
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    UbvG08 with I44A, P69L, and L70V mutations and no…

    Promoter

    CMV

    Available Since

    July 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #128587

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mortalin/GRP75 scFv [N52A/42]

    Plasmid

    #182091

    Purpose

    Mammalian Expression of Mortalin/GRP75 scFv. Derived from hybridoma N52A/42.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Mortalin/GRP75 (Mus musculus) (Hspa9 Mouse)
    

    Tags

    HA, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)

    Plasmid

    #114093

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548V and N552R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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