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pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Publication

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shBub1

Plasmid

#160948

Purpose

Bub1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Publication

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Bub1 shRNA (Bub1 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NES-tdTomato-LINuS-WPRE

Plasmid

#159894

Purpose

AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

Depositor

Tatsushi Onaka

Publication

Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

Insert

NES-tdTomato-LINuS

Use

AAV

Expression

Mammalian

Promoter

EF1α promoter

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134347

Plasmid

#152890

Purpose

Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046256

Plasmid

#153937

Purpose

Yeast expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4136081

Plasmid

#153935

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046327

Plasmid

#153913

Purpose

Yeast expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4252979

Plasmid

#153885

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046248

Plasmid

#153865

Purpose

Yeast expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133668

Plasmid

#153800

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133244

Plasmid

#153695

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046703

Plasmid

#149215

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046702

Plasmid

#149158

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046699

Plasmid

#149153

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046667

Plasmid

#149155

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046300

Plasmid

#149043

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046463

Plasmid

#145462

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp10

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PI3KC2alpha-PX (1405-1545)

Plasmid

#119119

Purpose

Bacterial expression of human phox homology (PX) domain, PI3KC2alpha-PX (1405-1545)

Depositor

Brett Collins

Publication

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

PI3KC2alpha-PX (1405-1545) (PIK3C2A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8.13-d

Plasmid

#136297

Purpose

expresses ABE8.13-d in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE8.13-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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PA-ecliptic pHluorin

Plasmid

#137837

Purpose

A photoactivatable GEVI variant originated from Bongwoori-R3

Depositor

Bradley Baker

Publication

Lee et al Front Cell Neurosci. 2019 Oct 29;13:482.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Photoactivatable-ecliptic pHluorin

Expression

Mammalian

Mutation

L64F, T65S, V163A, T203H

Promoter

CMV

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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