We narrowed to 17,871 results for: SHE

1. pBAD-SPW-RHH

   Plasmid

   #190630

   Purpose

   Bacterial expression of genetically encoded protease indicator SPW-RHH

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SPW-RHH
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Diacylglycerol Kinase Zeta

   Plasmid

   #85454

   Purpose

   expression of Diacylglycerol Kinase Zeta

   Depositor

   Stephen Gee

   Article

   Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

   Insert

   Diacylglycerol Kinase Zeta (DGKZ Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-ARP8

   Plasmid

   #15361

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   actin-related protein 8 (ACTR8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-hs_ICAM_full (JDW 1047)

   Plasmid

   #224479

   Purpose

   Gateway compatible 5' entry clone containing the Human ICAM2 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   ICAM2 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

   Plasmid

   #53225

   Purpose

   human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

   Depositor

   Jesse Rinehart

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Phosphoprotein synthesis

   Tags

   HIS and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli, both codons for Ser21…

   Promoter

   pLtetO

   Available since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-puro-tetON-shGFP

   Plasmid

   #110939

   Purpose

   Tet-inducible shRNA targeting GFP

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shGFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1/TO

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUBC_HA_PP7-mVenus

   Plasmid

   #111268

   Purpose

   Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   PP7-mVenus
   

   Use

   Synthetic Biology

   Tags

   HA, SV40 NLS, and mVenus

   Expression

   Mammalian

   Mutation

   PP7: C68S, S69G [delta]70-75 (non-aggregating)

   Promoter

   hUBC

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

   Plasmid

   #200910

   Purpose

   3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   3XHA_den1FnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Glutamic Acid to Arginine at 1369 position

   Promoter

   CBH

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CYFIP2.mCherry

   Plasmid

   #122052

   Purpose

   Expresses human CYFIP2 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP2 (CYFIP2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-Flag CASTOR1 I280A

    Plasmid

    #84495

    Purpose

    overexpression of CASTOR1 I280A mutant

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 I280A (GATSL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I280A

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCMV_eA3A-nCas9

    Plasmid

    #163535

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LP455

    Plasmid

    #90198

    Purpose

    pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 649-1426

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    NPHP4 (NPHP4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa residues 649-1426

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLHCX-Flag-mPKM2(C358S)

    Plasmid

    #42513

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 Cys358->Ser
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Changed Cysteine 358 to Serine

    Promoter

    CMV

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pPV-H1-ccdB-mEF1α-RiH

    Plasmid

    #100598

    Purpose

    piggyBac vector for sgRNA cloning

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Type

    Empty backbone

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human H1 PolIII promoter, human FTH1 promoter, mo…

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-human CSFI-R-L301S-IRES-GFP

    Plasmid

    #133744

    Purpose

    retroviral expression of CSF1-R mutant

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    CSF1-R (CSF1R Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L301S

    Promoter

    MSCV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LP453

    Plasmid

    #90184

    Purpose

    pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 1-839

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    NPHP4 (NPHP4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa residues 1-839

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35514

    Purpose

    Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArch3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-SuperNovaGreen-T2A-dTomato

    Plasmid

    #193798

    Purpose

    Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    SuperNova Green
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid

    #128404

    Purpose

    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A (toxA P. aeruginosa)
    

    Use

    Tags

    6xHis tag and DsbA signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3E-V5-mScarlet-I_SV40pA (JDW 968)

    Plasmid

    #224529

    Purpose

    A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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