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pBabe puro-HA-PIK3CA(H1047R;F486S;del)

Plasmid

#33255

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and phenylalan…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
pAdCMV/V5-DEST-S727D-STAT3-3xFlag

Plasmid

#99263

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (S727D)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - S727D (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

S727D

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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mIFP-Alpha-Actinin-19

Plasmid

#56210

Purpose

Localization: Focal Adhesions / Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-Vimentin-7

Plasmid

#57688

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Vimentin (VIM Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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actc1b-mKate2-SERCA1

Plasmid

#109498

Purpose

Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

SERCA1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Actin-7

Plasmid

#55085

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

Plasmid

#58275

Purpose

mammalian expression of human RdgBbeta splice variant 1

Depositor

Shamshad Cockcroft

Article

Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

Insert

RdgBbeta (PITPNC1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
miniSOG-Actin-7

Plasmid

#57761

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-his-Gluc-iLID

Plasmid

#172096

Purpose

Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - iLID

Expression

Bacterial

Mutation

Gluc Mutations - M43L, M110L

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-T0-Flag-HROB

Plasmid

#135298

Purpose

Expresses Flag-tagged HROB in mammalian cells

Depositor

Daniel Durocher

Article

Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

Insert

HROB (HROB Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDN-G1TGt

Plasmid

#44508

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
tetR::yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV10-mFoxP1-ES

Plasmid

#35169

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-MED11

Plasmid

#15418

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED11 (Med11 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGL-p21UTRm1&2

Plasmid

#20880

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Predicted miRNA binding site 1 (Position ~420) GC…

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
Rat Alpha2 delta-1 mutMIDAS pMT2

Plasmid

#58730

Purpose

expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells

Depositor

Annette Dolphin

Article

Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

MIDAS site mutations (D259VSGS to AVAGA)

Promoter

SV40

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPtprd#2/Cre

Plasmid

#173648

Purpose

Expresses a Ptprd-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ptprd (Ptprd Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPtprd#1/Cre

Plasmid

#173647

Purpose

Expresses a Ptprd-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ptprd (Ptprd Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta3-3xFiBsd

Plasmid

#140962

Purpose

full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis associated 3 (Mta3 Mouse, Synthetic)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1_sh_ex15

Plasmid

#35166

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5/BirA/FLAG-DDX54

Plasmid

#97063

Purpose

Expresses BirA/FLAG-tagged DDX54 in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

BirA and FLAG

Expression

Mammalian

Promoter

tetO2

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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