Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 11,589 results for: aga

Showing: 10721 - 10740 of 11589 results

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pLKO-U6-sgRXRa4-1; EF1a-mScarlet

Plasmid

#242889

Purpose

For lentiviral Crispr/Cas9 mediated knockout of mouse RXRa, using a guide RNA against exon 4, coupled to EF1a-driven mScarlet

Depositor

Elaine Fuchs

Article

Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:

Insert

sgRNA targeting Rxra

Use

CRISPR and Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_RPP14 (pAVA3507)

Plasmid

#239317

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP14

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting RPP14 (RPP14 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Col-0)

Plasmid

#229729

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) D332R

Plasmid

#229719

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-155 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) ΔC

Plasmid

#229717

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) Q345R

Plasmid

#229720

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, Q345R

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) D332R Q345R

Plasmid

#229721

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, D332R Q345R

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) S214A

Plasmid

#229723

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, S214A

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) T359A

Plasmid

#229727

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, T359A

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Hh-0)

Plasmid

#229728

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB512_35S-FLAG-(ΔTP)DM3(Hh-0) ΔC

Plasmid

#229730

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Col-0) ΔC

Plasmid

#229731

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-DM3(Col-0)68-510

Plasmid

#229733

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-67 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) Y460E V484E

Plasmid

#229715

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) D332A Q345A N349A

Plasmid

#229716

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, D332A Q345A N349A

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRIPK3_1k)-PGKpuro2ABFP-W

Plasmid

#208424

Purpose

Lentiviral gRNA plasmid targeting human RIPK3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RIPK3 (Ripk3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMTOR_1k)-PGKpuro2ABFP-W

Plasmid

#208429

Purpose

Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(WT)

Plasmid

#215450

Purpose

Gateway entry vector with integrin beta1 wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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sgYAP-R-1

Plasmid

#229445

Purpose

knockout of YAP1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-R-1 (YAP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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Cdk13_intron3_sg3_pX458

Plasmid

#127338

Purpose

sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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