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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHoY110_pAC8_hsDCAF5_FL

Plasmid

#249183

Purpose

Express DCAF5(full length) in Insect cells

Depositor

Eric Fischer

Article

Radko-Juettner et al Nature. 2024 Apr;628(8007):442-449. doi:

Insert

DCAF5 full length (DCAF5 Human)

Tags

Strep_BirA_short-linker-TEV

Expression

Insect

Mutation

N/A

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

Plasmid

#171630

Purpose

Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

Depositor

Chris French, Christopher A French

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

Insert

BRD4-NUT (BRD4, NUTM1 Human)

Tags

BioTAP

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpA

Plasmid

#163475

Purpose

Direct-expressing FlpO AAV Virus. Alias: AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

FlpO

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-BRD4

Plasmid

#23455

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BRD4 (BRD4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
AiP1011-pAAV-mscRE4-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163480

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1011 - pAAV-AiE2004m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

Plasmid

#114433

Purpose

Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6s, tTA2

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Calb1-T2A-dgCre targeting vector

Plasmid

#114438

Purpose

Target dgCre into the endogenous mouse Calb1 locus at the stop codon

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

dgCre

Use

Mouse Targeting

Tags

DHFR domain and EGFP

Promoter

none; utilizes endogenous Calb1 promoter for expr…

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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AiP981-pAAV-mscRE4-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163484

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP981 - pAAV-AiE2004m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

Plasmid

#114428

Purpose

Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6f

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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