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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,303 results for: crispr

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Showing: 10741 - 10760 of 25303 results

  1. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SA-GFP reporter
    Expression
    Mammalian
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  2. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA against mouse TTN for activation
    Expression
    Mammalian
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    128325-plasmid-map-sequence-id-255042

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  5. pLV-TET2BlastR-Cas9-TagBFP

    Plasmid
    #236726
    Purpose
    Dox Inducible Cas-TagBFP expression
    Depositor
    David Bersten
    Insert
    Cas9-TagBFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    May 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    236726-plasmid-map-sequence-id-474177

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  6. AICSDP-72:TFAM-mEGFP

    Plasmid
    #159745
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TFAM
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TFAM Homology Arms with linker-mEGFP (TFAM Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Oct. 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159745-plasmid-map-sequence-id-314375

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  9. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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  12. p3s-ABE7.10

    Plasmid
    #113128
    Purpose
    expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized
    Depositor
    Jin-Soo Kim
    Publication
    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE 7.10
    Tags
    HA epitope and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    113128-plasmid-map-sequence-id-216920

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  18. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Antonella Fidanza, Lesley Forrester
    Publication
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    211495-plasmid-map-sequence-id-454077

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Showing: 10741 - 10760 of 25303 results