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pPD_Pinx-1(1k + 50 bp)::Venus

Plasmid

#117421

Purpose

For analysis of AIB cell differentiation by tracking Venus fluorescence under the expression of Pinx-1 promoter

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

Venus

Expression

Worm

Promoter

inx-1

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pOEM1_pCMV:axFgf8-pPH:vsvged

Plasmid

#111159

Purpose

Baculovirus rescue vector, VSVGED pseudotype, hShh

Depositor

Elly Tanaka

Article

Nacu et al Nature. 2016 May 19;533(7603):407-10. do

Insert

axFgf8

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Mutation

wt

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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HM110 prp16-2

Plasmid

#111277

Purpose

prp16-2 in pSE358(Trp)

Depositor

Christine Guthrie

Article

Madhani et al Genetics. 1994 Jul;137(3):677-87.

Insert

PRP16

Expression

Yeast

Mutation

prp-2 (D575N)

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Yes-10kb

Plasmid

#112996

Purpose

P element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Xie et al Proc Natl Acad Sci U S A. 2008 Jul 15;10

Insert

10Kb DAFC-62D amplicon

Use

P insertion vector

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-RFP-miR-30 backbone

Plasmid

#97147

Purpose

As a control of miRNA expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-Flag-dTSC2 S1338A

Plasmid

#111808

Purpose

Insect Expression of dTSC2

Depositor

Jun Hee Lee

Article

Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

Insert

dTSC2 (TSC2 Fly)

Tags

3xFlag

Expression

Insect

Mutation

S1338A

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pKSJ375

Plasmid

#103064

Purpose

Expresses ImmE1, immunity to colicin E1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Insert

colicin E1 immunity protein (pColE1_26, imm E. coli)

Expression

Bacterial

Promoter

E1 immunity protein promoter from ColE1

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-pol30(K127R)

Plasmid

#99543

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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CTL2

Plasmid

#92212

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 of PIEZO1

Tags

6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;mafbbP1Egfp

Plasmid

#90163

Purpose

contains mafbb specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

mafbbP1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtK139A

Plasmid

#92403

Purpose

hQPRTase harbouring K139A mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV cleacable 6xHis

Expression

Bacterial

Mutation

K139A

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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p5EmafbbE1

Plasmid

#90114

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of mafbb

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

mafbbE1

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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LPhP-pHG165

Plasmid

#90047

Purpose

Constitutive expression of a chimeric LacI:PurR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

Insert

LPhP

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;nrp1bP1Egfp

Plasmid

#90164

Purpose

contains nrp1b specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

nrp1bP1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMEnrp1bP1gfp

Plasmid

#90129

Purpose

Gateway Mid-entry clone containing promoter sequence specific for nrpb1b driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

nrp1bP1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMEmafbbP1gfp

Plasmid

#90128

Purpose

Gateway Mid-entry clone containing promoter sequence specific for maffbb driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

maffbbP1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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p5Enrp1bE1

Plasmid

#90111

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of nrp1b

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

nrp1bE1

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pstb-LAFR5-exoY

Plasmid

#86170

Purpose

pstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned into Pst/Bam

Depositor

Kay Jones

Article

Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

Insert

exoY

Expression

Bacterial

Promoter

Smb21651

Available Since

Feb. 6, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX6P1-GST-Laforin C329S

Plasmid

#84682

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C278S

Plasmid

#84681

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only