We narrowed to 14,293 results for: SHI

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pFB2D

Plasmid

#195286

Purpose

Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

Plasmid

#203840

Purpose

Kir2.1 expression for silencing neurons and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Has Service

AAV8

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-monSTIM1

Plasmid

#161795

Purpose

Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

MonSTIM1 (STIM1 Human, Mustard Weed)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3/FKBP12-pBirA(79-C)-ha

Plasmid

#154449

Purpose

expresses FKBP12-pBirA(79-C)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

FKBP12-pBirA(79-C)-ha (FKBP12 Human)

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-EF1a-D614G-N501Y

Plasmid

#177939

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Expression

Mammalian

Mutation

D614G, N501Y

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ER-EBFP2

Plasmid

#231183

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK2-CR

Plasmid

#229421

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK2-CR (MARK2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

silent mutation CRISPR resistant

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEntr_Myc-BirA-G3-ER

Plasmid

#173814

Purpose

Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

Depositor

Norbert Perrimon

Article

Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

Insert

Myc-BirA-G3-ER

Use

Gateway shuttle vector

Tags

Myc

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-mLin28A

Plasmid

#26357

Depositor

George Daley

Article

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog A (Lin28a Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pIG-705pUC19-tNGFR-P2A-STAT5b

Plasmid

#186103

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

STAT5B (STAT5B Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-460 tNGFR-P2A-STAT4

Plasmid

#186075

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

STAT4 (STAT4 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_TRET_Ngn2‐2A‐Ngn1

Plasmid

#61471

Purpose

3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn2 (Neurog2 Mouse)
Neurog1 (Neurog1 Mouse)

Use

Lentiviral

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR wt

Plasmid

#214699

Purpose

Mammalian expression of human CALR wt

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR wt (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_UBXN4_R3HDM1

Plasmid

#205950

Purpose

Express mEGFP-tagged fusion protein, UBXN4_R3HDM1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

UBXN4_R3HDM1 (UBXN4, R3HDM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO_FOXA1_#2

Plasmid

#70096

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

u6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple

Plasmid

#214417

Purpose

Peroxisome localization of PMP34 fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

PMP34 (SLC25A17 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-P2A-mCherry

Plasmid

#204357

Purpose

AAV vector for miniDq expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-P2A-mCherry

Use

AAV

Tags

mCherry

Mutation

The third intracellular loop (ICL3) of hM3Dq was …

Promoter

hSyn

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TAK1/F

Plasmid

#44161

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-DN (S25N)

Plasmid

#101046

Purpose

Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Tags

HA

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-10nm-EBFP2

Plasmid

#231184

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

10nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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