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pCas-mut4_1_2_VA

Plasmid

#186441

Purpose

Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-4/1_2_VB

Plasmid

#186442

Purpose

Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-4/1Δ2_VB

Plasmid

#186443

Purpose

Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12(PI)

Plasmid

#186448

Purpose

Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12a (PI)

Use

CRISPR

Mutation

K613A, K617A

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12b

Plasmid

#186449

Purpose

Encodes Cas12b from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12b(PI)

Plasmid

#186451

Purpose

Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b (PI)

Use

CRISPR

Mutation

R122A, G143P

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmRoquin_702-819-del790-812-GB1His6_AE

Plasmid

#148623

Purpose

Bacterial Expression of DmRoquin_702-819-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del790-812 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-DmRoquin-del725-755-del790-812_AE

Plasmid

#148608

Purpose

Mammalian Expression of DmRoquin-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin-del725-755-del790-812 (roq Fly)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmRoquin_702-819-del725-755-GB1His6_AE

Plasmid

#148622

Purpose

Bacterial Expression of DmRoquin_702-819-del725-755

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del725-755 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmRoquin-del725-755-del790-812_AE

Plasmid

#148591

Purpose

Insect Expression of DmRoquin-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin-del725-755-del790-812 (roq Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1467-1717_AE

Plasmid

#148598

Purpose

Insect Expression of DmNot1_1467-1717

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmNot1_1467-1717 (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4_275-376-GB1His6_AG

Plasmid

#148784

Purpose

Bacterial Expression of HsNot4_275-376

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-376 (CNOT4 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4_275-575-GB1His6_AG

Plasmid

#148788

Purpose

Bacterial Expression of HsNot4_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575 (CNOT4 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4_377-428-GB1His6_AG

Plasmid

#148789

Purpose

Bacterial Expression of HsNot4_377-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_377-428 (CNOT4 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4_400-424_AG

Plasmid

#148790

Purpose

E. coli of HsNot4_400-424

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_400-424 (CNOT4 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4_429-575-GB1His6_AG

Plasmid

#148791

Purpose

Bacterial Expression of HsNot4_429-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_429-575 (CNOT4 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-376_AG

Plasmid

#148751

Purpose

Mammalian Expression of HsNot4_275-376

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-376 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575-FFTLtoDAEE_AG

Plasmid

#148756

Purpose

Mammalian Expression of HsNot4_275-575-FFTLtoDAEE

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-FFTLtoDAEE (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_429-575_AG

Plasmid

#148758

Purpose

Mammalian Expression of HsNot4_429-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_429-575 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNot4-del377-424_AG

Plasmid

#148760

Purpose

Mammalian Expression of HsNot4-del377-424

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del377-424 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge