We narrowed to 2,122 results for: D10

1. pJMP8823

   Plasmid

   #220901

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. dead Sa Cas9

   Plasmid

   #138162

   Purpose

   For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   dSaCas9
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D10A + N580A

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_256

   Plasmid

   #158581

   Purpose

   Lentiviral expression of BE3.9max and customizable gRNA

   Depositor

   John Doench, David Root

   Article

   Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_dCas9-KRAB-MeCP2_Blast

   Plasmid

   #221501

   Purpose

   Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   dCas9-KRAB-MeCP2-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-BE-zCas9

   Plasmid

   #101739

   Purpose

   expression of an optimized Cas9 for base-editing in zebrafish

   Depositor

   Shuo Lin

   Article

   Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

   Insert

   rAPOBEC1-XTEN-zCas9-UGI-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMV

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hUbC-dCas9-T2A-GFP

   Plasmid

   #53191

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9-J23111

   Plasmid

   #113149

   Purpose

   Plasmid constitutively expressing dCas9 protein

   Depositor

   Chong Zhang

   Article

   Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A & H840A

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

   Plasmid

   #105234

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Promoter

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pScI_dCas9-CDA-UL

   Plasmid

   #108551

   Purpose

   Bacterial Target-AID UGI-LVA vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   dCas9-PmCDA1-UGI-LVA
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   lambda OR

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMJ841

    Plasmid

    #39318

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    T7

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. SlugABEmax

    Plasmid

    #163798

    Purpose

    Expresses ABEmax and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Promoter

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMP8930

    Plasmid

    #220905

    Purpose

    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMJ825

    Plasmid

    #39315

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A)

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xFLAG-dCas9/p-bacteria

    Plasmid

    #64325

    Purpose

    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR; Tetracycline-inducible

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    pLtetO-1

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. ePPE-spG

    Plasmid

    #183096

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nspGCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpGCas9

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

    Plasmid

    #192650

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHEE901

    Plasmid

    #91707

    Purpose

    CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV2-CAGIG-LAMP1-mGL

    Plasmid

    #222394

    Purpose

    To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.

    Depositor

    Pantelis Tsoulfas

    Insert

    LAMP1 and mGreenlantern (LAMP1 Synthetic, Homo Sapiens, Human)
    

    Use

    AAV

    Tags

    LAMP1 and mGreenLantern

    Expression

    Mammalian

    Mutation

    Promoter

    CAG or CAGIG

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF_dCas9-VP64

    Plasmid

    #68417

    Purpose

    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and VP64

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV4-p100

    Plasmid

    #23287

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    NFkB p100 subunit (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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