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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,203 results for: LEA

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Showing: 10781 - 10800 of 12203 results

  1. D282N Cus2p

    Plasmid
    #73060
    Purpose
    S. cerevisiae Cus2p gene with D282N Mutation
    Depositor
    Aaron Hoskins
    Publication
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Asp 282 to Asn
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    73060-plasmid-map-sequence-id-150798

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  4. pJK520

    Plasmid
    #155386
    Purpose
    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Availability
    Academic Institutions and Nonprofits only
    155386-plasmid-map-sequence-id-309003

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  6. SpyTag-mGreenLantern-A OR T OR C-SpyTag

    Plasmid
    #250641
    Purpose
    Expresses crosslink enabling OROR-gated degradation and release of mGL from materials in response to eSrtA(2A9) OR TEV OR HRV-3C treatment. Construct contains two SpyTags for material creation.
    Depositor
    Cole DeForest
    Publication
    Ross et al Adv Mater. 2026 Jul 21:e74038. doi: 10.1
    Insert
    MGSS-SpyTag-GGS(x3)-mGreenLantern-GGS(x3)-2A9c-GGS(x3)-TEVc-GGS(x3)-HRV-3Cc-GGS(x3)-SpyTag-GGS(x3)-6xHis
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Aug. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    250641-plasmid-map-sequence-id-497423

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  7. SpyTag-MBP-A OR T OR C-SpyTag

    Plasmid
    #250648
    Purpose
    Expresses crosslink enabling OROR-gated degradation and release of MBP from materials in response to eSrtA(2A9) OR TEV OR HRV-3C treatment. Construct contains two SpyTags for material crosslinking.
    Depositor
    Cole DeForest
    Publication
    Ross et al Adv Mater. 2026 Jul 21:e74038. doi: 10.1
    Insert
    MGSS-SpyTag-GGS(x3)-MBP-GGS(x3)-2A9c-GGS(x3)-TEVc-GGS(x3)-HRV-3Cc-GGS(x3)-SpyTag-GGS(x3)-6xHis
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Aug. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    250648-plasmid-map-sequence-id-497416

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  13. pYSD726

    Plasmid
    #253049
    Purpose
    Encodes OST1 Sig Pep, LC Cutinase-ICCG variant, FLAG-tagged SED1 surface display anchor in a yeast expression vector with pTEF1 promoter, tTDH1 term, LEU2 selectable marker and CEN/ARS origin
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Leaf compost cutinase
    Expression
    Yeast
    Mutation
    Y127G, D238C, F243I, S283C
    Available since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253049-plasmid-map-sequence-id-508689

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  14. JEV-ED3_C5I

    Plasmid
    #251260
    Purpose
    To express the Japanese encephalitis virus envelope protein domain III with a five-isoleucine tag at the C-terminus in E. coli
    Depositor
    Yutaka Kuroda
    Publication
    Islam et al Vaccine. 2025 May 22;56:127143. doi: 10.
    Insert
    JEV-ED3_C5I
    Tags
    5xIle with a GG linker and 6xHis, 11 aa linker, t…
    Expression
    Bacterial
    Available since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    251260-plasmid-map-sequence-id-508114

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  19. pAvA141

    Plasmid
    #248146
    Purpose
    mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations
    Depositor
    Emil Jensen
    Publication
    van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s
    Insert
    luxR (gen2 mutations)
    Use
    Integration vector
    Tags
    Gal4 activation domain and nuclear localization s…
    Expression
    Yeast
    Mutation
    Gal4_AD: G8V. NLS: K120N. LuxR: N86K, K104E, M135V
    Promoter
    pPGK1
    Available since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248146-plasmid-map-sequence-id-497175

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  20. pZucchini2_SFFV-RXRA-eGFP-IRES-mCherry_PuroR

    Plasmid
    #247929
    Purpose
    Overexpression vector for eGFP tagged RXRA or NR2B1. Monitor post-translational degradation of RXRA via EGFP:mCherry ratio. Contains puromycin-resistance selection marker.
    Depositor
    Benjamin Ebert, Jonathan Tsai
    Publication
    Tsai et al Mol Cell. 2023 Aug 3;83(15):2753-2767.e1
    Insert
    Retinoid X receptor alpha (RXRA Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247929-plasmid-map-sequence-id-496526

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Showing: 10781 - 10800 of 12203 results