We narrowed to 16,451 results for: LEA
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Plasmid#152050PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable SinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)
Plasmid#211861PurposeNuclear-localized E3-DART ligase in domestication vector.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HAAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
438-GST
Plasmid#97092PurposeGST-tev-yORFDepositorTypeEmpty backboneTagsGST tag and TEV cleavage siteExpressionInsectPromoterPolyhedrin promoterAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p(bhsp68-Cas9)
Plasmid#65959PurposeTribolium basal heat shock promoter driving Cas9DepositorInsertCas9
UseCRISPRTagsnuclear localization sequenceExpressionInsectPromoterbhspAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB4280
Plasmid#98699PurposeA Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CBX7
Plasmid#25241PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
NLS-LgBiT-CaM
Plasmid#183401PurposeExpresses nuclear-localized LgBiT-CaMDepositorInsertLgBiT-CaM
TagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_hH1_mEos3.1_V5
Plasmid#191750PurposePhotoconvertible fluorescent nuclear reporter.DepositorAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICXNHG3
Plasmid#49021PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage siteDepositorTypeEmpty backboneTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastPromoterAOX1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLICER
Plasmid#197288PurposeConjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transferDepositorInsertI-SceI Endonuclease
UseSynthetic Biology; Bacterial and yeast cloningAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBHPS-SortA
Plasmid#21930DepositorInsertsortase
TagsGB1, HRV3C cleagage site, and HisExpressionBacterialMutationdeleted amino acids 1-59Available SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133227
Plasmid#152132PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable SinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPV-DsRed2-ER-5
Plasmid#170571PurposeExpresses DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.DepositorInsertEPV-DsRed2-ER-5
ExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2108
Plasmid#201969PurposeORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.DepositorInsertattB-3C-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pE_Cre_lambdaCos
Plasmid#173785PurposeThe pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.DepositorInsertCre
UseCre/Lox and Synthetic BiologyMutationS102Y and S279Y- please see depositor commentsAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEH275 NLS dTomato
Plasmid#168210PurposeMammalian expression of nuclear-localized dTomatoDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-LaminA-N-18
Plasmid#54139PurposeLocalization: Nuclear Envelope, Excitation: 487, Emission: 509DepositorAvailable SinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits