We narrowed to 11,592 results for: Vars;

1. pENTR1A mKate2-hu_ncOGT D554N

   Plasmid

   #154283

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Mutation

   D554N mutation (Ser/Thr Glycosylation Impaired)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDN-T2dMFot

   Plasmid

   #44558

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

   Inserts

   PTETREG promoter
   rtetR-M2::FFF
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Expression

   Yeast

   Mutation

   Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

   Promoter

   PTETREG

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR1A mKate2-hu_ncOGT 5N5A

   Plasmid

   #154285

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations (HCF-…

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 3322_pETcon_SARS2_Omicron-BA2

   Plasmid

   #184409

   Purpose

   yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. P2_HNF4A8

   Plasmid

   #31094

   Depositor

   Gerhart Ryffel

   Article

   Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   splice variant 8 derived from promoter P2

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-syn-FLEX-jYCaMP1s

   Plasmid

   #135420

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

   Plasmid

   #111387

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

   Plasmid

   #114099

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1

   Plasmid

   #101177

   Purpose

   Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FR_HNF4A2-C106R

    Plasmid

    #31123

    Depositor

    Gerhart Ryffel

    Article

    Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

    Insert

    HNF4A (HNF4A Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 2, mutation C106R. Wild type seq…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114104

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    sbGLuc-VChR1-EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFLAG-EEF1D (pcDNA 3.1+) LG3-1

    Plasmid

    #37364

    Depositor

    David Litchfield

    Article

    Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

    Insert

    EEF1D (EEF1D Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

13. CMV-218_HNF4A2

    Plasmid

    #31090

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 2 of HNF4A, deletion of CMV promo…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-PE NG

    Plasmid

    #174554

    Purpose

    All-in-one prime editor piggyBac transposon, NG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

    Promoter

    CAG

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-138_HNF4A8

    Plasmid

    #31093

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 8 of HNF4A, deletion of CMV promo…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-68_HNF4A2

    Plasmid

    #31092

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 2 of HNF4A, deletion of CMV promo…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

    Plasmid

    #184407

    Purpose

    yeast surface display of the SARS-CoV-2 Delta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    L452R+T478K

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV-138_HNF4A2

    Plasmid

    #31091

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 2 of HNF4A, deletion of CMV promo…

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCR-HA-IKKalphad17

    Plasmid

    #19738

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase alpha (Chuk Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Splice variant lacking 17 amino acids at the C-te…

    Available Since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pU6-crRNA(TBK1)

    Plasmid

    #109322

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (TBK1 Human)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge