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KTK_029

Plasmid

#180542

Purpose

Entry vector containing RBS BBa_B0035

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

RBS BBa_B0035

Expression

Bacterial

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_034

Plasmid

#180526

Purpose

Entry vector containing pLux promoter

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pLux promoter

Expression

Bacterial

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_035

Plasmid

#180557

Purpose

Entry vector containing L3S1P00 terminator

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P00 terminator

Expression

Bacterial

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_033

Plasmid

#180545

Purpose

Entry vector containing LuxR

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

LuxR

Expression

Bacterial

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_037

Plasmid

#180559

Purpose

Entry vector containing L3S1P22 terminator

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P22 terminator

Expression

Bacterial

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_003

Plasmid

#180541

Purpose

Entry vector containing RBS BBa_B0034

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

RBS BBa_B0034

Expression

Bacterial

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_039

Plasmid

#180561

Purpose

Entry vector containing L3S2P21 terminator

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P21 terminator

Expression

Bacterial

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_038

Plasmid

#180560

Purpose

Entry vector containing L3S2P11 terminator

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P11 terminator

Expression

Bacterial

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAT.334

Plasmid

#119556

Purpose

Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.007

Plasmid

#119671

Purpose

Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Pcpc560-Dx2

Use

Synthetic Biology

Mutation

bp 89 T to C, bp 187 C to A, Duplicated downstrea…

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.006

Plasmid

#119670

Purpose

Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Pcpc560-Ux2

Use

Synthetic Biology

Mutation

bp 89 T to C, bp 187 C to A, Duplicated upstream …

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.074

Plasmid

#119663

Purpose

Level 0 part. Terminator from Cysteine synthase A gene (cysK) from E. coli.

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120048902

Use

Synthetic Biology

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.073

Plasmid

#119660

Purpose

Level 0 part. Terminator from rplJL-rpoBC,rplKAJL-rpoBC,rpoBC gene cluster from E. coli.

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010869

Use

Synthetic Biology

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.070

Plasmid

#119652

Purpose

Level 0 part. Terminator, Bi-directional terminator for yciA/tonA genes from E. coli.

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

BBa_B0061

Use

Synthetic Biology

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

Plasmid

#121825

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/p300core (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

Plasmid

#121826

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/Dnmt3a-3L (open)

Use

Gateway Cloning and Synthetic Biology

Tags

NLS

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

Plasmid

#121836

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

Plasmid

#121837

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

Plasmid

#121838

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KO602: pMVP (L3-L2) HA tag + pA; CMV::TETa-pA

Plasmid

#121802

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven TETa

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + CMV::TETa-polyA

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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