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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 60,382 results for: cloning

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Showing: 10821 - 10840 of 60382 results

  1. HRE:GFP-pGK:Cre

    Plasmid
    #68552
    Purpose
    Lentivirus expressing hypoxia-responsive GFP reporter and Cre
    Depositor
    Tyler Jacks
    Inserts
    Green Fluorescent Protein
    Cre
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    HRE and pGK
    Available Since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    68552-plasmid-map-sequence-id-120714

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  2. mCherry-Rab5C-S35N

    Plasmid
    #110503
    Purpose
    Dominant Negative
    Depositor
    Bernardo Mainou
    Insert
    Rab5C-S35N (RAB5C Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    S35N
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110503-plasmid-map-sequence-id-210729

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  3. pCAG-N2cG

    Plasmid
    #100811
    Purpose
    express rabies virus CVS N2c strain glycoprotein gene (N2cG)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain glycoprotein gene
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    100811-plasmid-map-sequence-id-189508

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  4. HT7-mTurquoise2-KDEL

    Plasmid
    #188571
    Purpose
    Expresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    HaloTag7
    Tags
    KDEL and mTurquoise2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    188571-plasmid-map-sequence-id-369501

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  5. Nup133_mEGFP(-8a)

    Plasmid
    #163417
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163417-plasmid-map-sequence-id-321553

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  6. pExp-Sumo

    Plasmid
    #112574
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112574-plasmid-map-sequence-id-217243

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  7. pmr101A-cPr-mCherry (ErmR)

    Plasmid
    #177206
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    Synthetic constitutive promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177206-plasmid-map-sequence-id-348606

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  9. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  10. pSV40-mCherry-P2A-Hygro

    Plasmid
    #106478
    Purpose
    Expression of mCherry and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    mCherry
    Hygromycin B resistance
    Expression
    Mammalian and Plant
    Promoter
    SV40 promoter
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106478-plasmid-map-sequence-id-207986

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  11. RARS_pNIC-Bio3

    Plasmid
    #153055
    Purpose
    Expression construct for producing Avi tagged Arginyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    RARS (RARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153055-plasmid-map-sequence-id-304475

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  12. pRetroQ-Cre-ERT2

    Plasmid
    #59701
    Purpose
    to make Cre-ERT2 expressing stable cell line by retrovirus
    Depositor
    Richard Youle
    Insert
    Cre-ERT2
    Use
    Retroviral
    Tags
    Estrogen receptor (ERT)
    Expression
    Mammalian
    Promoter
    cmvIE
    Available Since
    Sept. 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    59701-plasmid-map-sequence-id-93474

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  13. pExp-NHis

    Plasmid
    #112558
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112558-plasmid-map-sequence-id-217235

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  14. DH-BB(5'SIN;TE12ORF)

    Plasmid
    #72309
    Purpose
    Defective Helper to produce neurotropic, propagation-incompetent Sindbis virus
    Depositor
    Anthony Zador
    Insert
    DH-BB(5'SIN;TE12ORF)
    Use
    Sindbis virus helper construct
    Available Since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72309-plasmid-map-sequence-id-145921

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  16. pGMC00010 (aka pRC0495)

    Plasmid
    #195295
    Purpose
    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195295-plasmid-map-sequence-id-387224

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  17. PG1424PGA

    Plasmid
    #74653
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PG1424PGA
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. TARS_pNIC-Bio3

    Plasmid
    #153057
    Purpose
    Expression construct for producing Avi tagged Threonyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    TARS (TARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153057-plasmid-map-sequence-id-304477

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  19. PA-mCherry1-C1

    Plasmid
    #54495
    Purpose
    Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54495-plasmid-map-sequence-id-218682

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  20. pBP-P_tetR-PTet*

    Plasmid
    #204077
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    tetR repressor and PTet* tetracycline-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204077-plasmid-map-sequence-id-413892

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Showing: 10821 - 10840 of 60382 results