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pGEX-PGC1(1-400)

Plasmid

#1029

Depositor

Bruce Spiegelman

Publication

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains only aa1-400

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRS303-LacZ

Plasmid

#1169

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

LACZ

Tags

GAL1:10 promoter

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

Plasmid

#128608

Purpose

Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Expression

Mammalian

Promoter

Pcmv

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Publication

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CRISPR-SB

Plasmid

#177936

Purpose

Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

Depositor

Roland Rad

Publication

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

CRISPR; Transposon

Expression

Mammalian

Promoter

U6

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD3

Plasmid

#114126

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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PB-TAC-ERP2-(N-HA)KRAS_G12D

Plasmid

#201254

Purpose

Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

Depositor

Alexander Kleger

Publication

Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

Insert

KRAS (KRAS Human)

Use

Piggybac transposon

Tags

HA-Tag

Expression

Mammalian

Mutation

changed Gly (G) 12 to Asp (D)

Promoter

TRE

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1199

Plasmid

#29100

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple53 (DCX Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_BiPVe4_GCaMP6f

Plasmid

#213939

Purpose

AAV construct expressing GCaMP6f driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_GCaMP6f

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV PHP.eB and AAV1

Insert

GCaMP6f

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-S473A

Plasmid

#49190

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

Depositor

Peter Vogt

Publication

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

S473A

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
GAL 46Q+ProGFPp416

Plasmid

#15581

Depositor

Susan Lindquist

Publication

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q + Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-CaMKIIa-mScarlet (AAV8)

Viral prep

#131000-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-mScarlet (#131000). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mScarlet plasmid DNA. CamKIIa-driven expression of mScarlet. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Publication

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Promoter

CaMKII

Tags

mScarlet

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-YPT7

Plasmid

#18847

Depositor

Susan Lindquist

Publication

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT7 (YPT7 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTubulin-Electra2

Plasmid

#184930

Purpose

Tubulin labeling with blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

TUBA1B-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6-mTFP1-KanMX6

Plasmid

#69851

Purpose

C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Publication

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Expression

Yeast

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Julien Berro, Thomas Pollard

Publication

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Expression

Bacterial and Yeast

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV-hygro-PAX9

Plasmid

#75083

Purpose

[Edit] retroviral vector holding human PAX9 cDNA

Depositor

David Mu

Publication

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

full length, wild type, without native 3' UTR

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-CK(0.4)GW

Plasmid

#27226

Depositor

Pavel Osten

Publication

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

AAV

Tags

EGFP

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMSSM24: pCMV-SpuFz1

Plasmid

#205260

Purpose

Human expression vector for human codon optimized SpuFz1

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Expression

Mammalian

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Publication

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only