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iPE-D10A-Cas9

Plasmid

#239380

Purpose

For inverse prime editor using nCas9-D10A in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9-D10A, M-MLV RT

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D10A in Cas9

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-TMSB10

Plasmid

#191133

Purpose

expresses TMSB10 in mammalian cells

Depositor

Ken-ichirou Morohashi

Article

Inoue et al Commun Biol. 2022 Sep 15;5(1):974. doi:

Insert

Thymosin beta 10

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSE152: pFuGW-hUbCp-3CLpro-SV40NLS

Plasmid

#240455

Purpose

SARS-CoV-2 Protease 3CLpro expression plasmid

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Insert

3CLpro

Use

Lentiviral

Promoter

hUbCp

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW10K_0x5

Plasmid

#177278

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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p-HITI-GFP-Ckm

Plasmid

#226119

Purpose

HITI insert construct with Ckm gRNA and GFP transgene

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Insert

Ckm (Ckm Mouse)

Use

CRISPR

Tags

eGFP

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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D10A-Cas9

Plasmid

#239383

Purpose

For circular RNA-mediated inverse prime editor using nCas9-D10A in HEK294T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9-D10A

Use

CRISPR

Tags

BPNLS and SV40 NLS

Expression

Mammalian

Mutation

D10A in Cas9

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1625

Plasmid

#239834

Purpose

OrufIscB-REC E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-REC

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Insertion of REC domain from NbaCas9-1

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1505

Plasmid

#239830

Purpose

OrufIscB-REC mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-REC

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS794

Plasmid

#238207

Purpose

Expresses native TaTasR TIGR locus, TasR has premature stop codon. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Mutation

A insertion at K85 codon

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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PC2328

Plasmid

#232089

Purpose

Mammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.

Depositor

Pranam Chatterjee

Article

Hong et al Nat Commun. 2025 Apr 15;16(1):3555. doi:

Insert

duAb with cloning Esp3I restriction site

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-cCGN_D-Myc

Plasmid

#236525

Purpose

For expression of Chimera of B-region cCGN in cCGNL1 in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGNL1-CGN (CGN Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-cCGNL1_D-Myc

Plasmid

#236523

Purpose

For expression of Chimera of D-region cCGNL1 in cCGN in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGN-CGNL1 (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-cCGNL1_B-Myc

Plasmid

#236522

Purpose

For expression of Chimera of B-region cCGNL1 in cCGN in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGN-CGNL1 (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-cCGN_B-Myc

Plasmid

#236524

Purpose

For expression of Chimera of D-region cCGN in cCGNL1 in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGNL1-CGN (CGN Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-hPLEKHA6-Myc

Plasmid

#236540

Purpose

For expression of PLEKHA6 (human) in mammalian cells

Depositor

Sandra Citi

Article

Sluysmans et al Mol Biol Cell. 2021 Nov 1;32(21):ar34. d

Insert

PLEKHA6 (PLEKHA6 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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1010Av2

Plasmid

#125642

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Expression

Insect

Promoter

10xUAS

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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1010Bv2

Plasmid

#125643

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Expression

Insect

Promoter

10xUAS

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-ImpBeta1-mEosEM

Plasmid

#218933

Purpose

Express ImpBeta1 (human) with mEosEM fused at the C-terminus.

Depositor

Siegfried Musser

Article

Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

Insert

Importin Beta (KPNB1 Human)

Tags

6xHis and mEos4b (EosEM)

Expression

Bacterial

Promoter

T7

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_RFP_WPRE_polyA

Plasmid

#220222

Purpose

AAV-STARR-seq validation vector with RFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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RhoA gRNA #1

Plasmid

#198718

Purpose

Cas9-mediated knockout of RhoA in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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