We narrowed to 15,822 results for: LEA

1. Parp14-c011

   Plasmid

   #173098

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K791-F978

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV10-3xFLAG-prs-XK

   Plasmid

   #184369

   Purpose

   Express human XK in Expi293 cells

   Depositor

   Karin Reinisch

   Article

   Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

   Insert

   Human XK (XK Human)
   

   Use

   Tags

   3xFLAG-PreScission cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. NES-mTagBFP2-P4Mx1

   Plasmid

   #108142

   Purpose

   PI4P biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   X.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. TARG1-c002

   Plasmid

   #173108

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   TARG1
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   Full length protein

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCW02

   Plasmid

   #72301

   Purpose

   Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Type

   Empty backbone

   Use

   Derivative of puc19

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5KO9 (HMCES)

   Plasmid

   #101877

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HMCES (HMCES Human)
   

   Use

   Tags

   6x His (modified C-term tag is AENLYFQSHHHHHH)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pME-GFP-P2A

   Plasmid

   #80807

   Purpose

   GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   GFP-P2A
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. csr-1_son-1_mNeonGreen

   Plasmid

   #191743

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mNeonGreen

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. zCD2

   Plasmid

   #122031

   Purpose

   Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

   Insert

   Zebrafish HDAC6 Catalytic Domain 2
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS1938

    Plasmid

    #82563

    Purpose

    High copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

    Insert

    Ple261
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pETNb-nALFA

    Plasmid

    #159988

    Purpose

    For the protein expression with ALFA tag at N-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET26b_Nb.AT110.i1

    Plasmid

    #135481

    Purpose

    E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110.i1
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid

    #216286

    Purpose

    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ⍺-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST14-DoubleCatcher-β-Lock

    Plasmid

    #216287

    Purpose

    Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher β-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST14-DoubleCatcher-γ-Lock

    Plasmid

    #216288

    Purpose

    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher γ-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST14-DoubleCatcher-δ-Lock

    Plasmid

    #216289

    Purpose

    Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher δ-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST14-DoubleCatcher-ε-Lock

    Plasmid

    #216290

    Purpose

    Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ε-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJCC_083 Cas12i1

    Plasmid

    #171670

    Purpose

    For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6CCR (GID4)

    Plasmid

    #119960

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Use

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 116-300

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. KLHL3 (KLHL3A-c003)

    Plasmid

    #110251

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL3 (KLHL3 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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