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pFUS_B7

Plasmid

#31024

Depositor

Adam Bogdanove, Daniel Voytas

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pFUS_B8

Plasmid

#31025

Depositor

Adam Bogdanove, Daniel Voytas

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pFUS_B9

Plasmid

#31026

Depositor

Adam Bogdanove, Daniel Voytas

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG2-GCaMP6s

Plasmid

#241022

Purpose

An AAV transfer plasmid expressing the GCaMP6s genetically encoded calcium indicator under control of the ubiquitously expressed CAG2 promoter.

Depositor

Thomas Johnson

Publication

Aguzzi et al Sci Transl Med. 2026 Apr 29;18(847):eadr

Insert

GCaMP6s

Use

AAV

Tags

6X His tag

Promoter

CAG2

Available since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pPICZ:hTRAAK(W262S)

Plasmid

#130670

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Publication

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, W262S

Available since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI (JDW 1494)

Plasmid

#242592

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag

Plasmid

#179721

Purpose

Lentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Publication

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

Plasmid

#230990

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Publication

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Cre-dependent, membrane-localized Voltron2 and soma-localized CheRiff

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Strep-FLAG KBP

Plasmid

#236459

Purpose

transient overexpression of KBP in mammalian cells

Depositor

Michele Pagano

Publication

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

KBP (KIF1BP Human)

Tags

Strep-FLAG

Expression

Mammalian

Available since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-SEC61B-mCherry-FRB

Plasmid

#233349

Purpose

Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

Depositor

Noboru Mizushima

Publication

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

SEC61B (SEC61B Human)
mCherry
FRB

Use

Retroviral

Expression

Mammalian

Mutation

T2098L mutant of FKBP-rapamycin binding domain of…

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OMM-short-RspA-NFAST

Plasmid

#233603

Purpose

Expression of RspA-NFAST on the outer mitochondrial membrane

Depositor

Riccardo Filadi

Publication

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RspA-NFAST

Expression

Mammalian

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Dre-nls (JDW 1131)

Plasmid

#229809

Purpose

A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DRE recombinase-nls

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-DEST (JDW 1186)

Plasmid

#229820

Purpose

An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

AAV

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-TCAB1 KI sgRNA

Plasmid

#207611

Purpose

sgRNA for HaloTag insertion into the endogenous TCAB1 locus

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCAAAGTCTTCATACTGCAG

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[I349D]-His (SB256)

Plasmid

#227605

Purpose

Inducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastoris

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Mutation

I349D

Promoter

AOX1

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EFS (EF1a core) (JDW 1319)

Plasmid

#224474

Purpose

Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS promoter

Expression

Mammalian

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-ALFA-CD28

Plasmid

#223630

Purpose

Lentiviral expression of human CD28 with ALFA tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

ALFA tag

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28

Plasmid

#223622

Purpose

Lentiviral expression of human CD28 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pert Light chain

Plasmid

#216310

Purpose

Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Pert Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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