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R701-X24-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R

Plasmid

#159573

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61R

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X23-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L

Plasmid

#159572

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61L

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X58-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R

Plasmid

#159566

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61R

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PRDX5K85R-VA

Plasmid

#98691

Purpose

Lentiviral expression of human PRDX5-K85R in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

K85R

Promoter

CMV

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PRDX5E80K-VA

Plasmid

#98689

Purpose

Lentiviral expression of human PRDX5-E80K in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

E80K

Promoter

CMV

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PRDX5K83R-VA

Plasmid

#98690

Purpose

Lentiviral expression of human PRDX5-K83R in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

K83R

Promoter

CMV

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-30)

Plasmid

#199167

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(-30)

Tags

MGHHHHHHGGA

Mutation

T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAR-Ec318

Plasmid

#130871

Purpose

A nuclear plasmid encoding the WT TP-DNAP1 for OrthoRep

Depositor

Chang Liu

Article

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-T-Sapphire

Plasmid

#103977

Purpose

Plasmid encoding T-Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

T-Sapphire

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (Q69M, C70V, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-GFP

Plasmid

#83375

Purpose

Expression of Myc tagged GFP

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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UIMLx2

Plasmid

#174888

Purpose

UIMLx2 ubiquitin probe for K48 ubiquitylated proteins

Depositor

Peter Kaiser

Article

Villamil et al Mol Cell Proteomics. 2022 Jan;21(1):1001

Insert

Met4 UIML

Tags

Avi-6xHis-Smt3

Expression

Bacterial

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Martine Roussel, Peter ten Dijke

Article

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Expression

Mammalian

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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p177bp-LacOx50-Hyg

Plasmid

#210213

Purpose

Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMVV094

Plasmid

#215302

Purpose

Expresses sfGFP under the Anderson J23119 promoter with a pRO1600 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

J23119

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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HRE-oUnaG

Plasmid

#201712

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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yWL268 MCP-GFP

Plasmid

#197052

Purpose

pUBC-NLS-HA-2xMCP-2xGFP

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-2xGFP

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJB06

Plasmid

#190480

Purpose

Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Cas9
XylR

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Promoter

xylR-driven promoter

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJB07

Plasmid

#190481

Purpose

Targeting plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Upstream & Downstream pyrE deletion region
gRNA

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-CAXX

Plasmid

#184451

Purpose

Plasma membrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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