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pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

Plasmid

#169332

Purpose

Nuclear expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag9 (H2BC21 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre stuffer v3

Plasmid

#158030

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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3xFLAG-SENP7

Plasmid

#42886

Depositor

Edward Yeh

Article

Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

Insert

SENP7 (SENP7 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

T102A, R969C, L996S

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pV1326

Plasmid

#111435

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry_sgRNA-ver2

Plasmid

#126776

Purpose

gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

sgRNA (for SpCas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S in pAR9 (rep/cap)

Plasmid

#174539

Purpose

encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

Depositor

Casey Maguire

Article

Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

Insert

VP1 of AAV9 containing the AAV-S 7-mer peptide insert

Use

AAV

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES

Plasmid

#124707

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAB107a

Plasmid

#79218

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

Plasmid

#197989

Purpose

For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNT0217290

Use

Lentiviral

Tags

lac

Expression

Mammalian

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES

Plasmid

#124703

Purpose

Expresses MS2_adRNA targeting the RAB7A locus along with MCP_ADAR1 DD (E1008Q)_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E1008Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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