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Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA1-gltA2
Plasmid#71348PurposegltA1-gltA2 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA1-gltA2 guide array
ExpressionBacterialPromoterE . coli ugpB gene promoter, low phosphate induct…Available SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK_lacO-cP-AOX1-AtX+NpgA
Plasmid#126727Purposeyeast plasmid overexpressing AtX and NpgADepositorInsertatX,npgA
Tags6xHisExpressionYeastAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG_P-HXT1-FAS1up
Plasmid#126747Purposevector for replacing the promoter of FAS1 coding geneDepositorInsertfas1 upstream
Tags6xHisExpressionYeastPromoterHXT1Available SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2
Plasmid#126734Purposeyeast plasmid overexpressing ADH2 from Saccharomyces cerevisiaeDepositorInsertadh2
Tags6xHisExpressionYeastPromoterGAPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_-P-GAP-NpgA+AtX
Plasmid#126725Purposeyeast plasmid overexpressing AtX and NpgADepositorInsertatX,npgA
Tags6xHisExpressionYeastPromoterGAPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-LovC
Plasmid#126729Purposeyeast plasmid overexpressing LovCDepositorInsertlovC
Tags6xHisExpressionYeastAvailable SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-LovB
Plasmid#126728Purposeyeast plasmid overexpressing LovBDepositorInsertlovB
Tags6xHisExpressionYeastMutationT7227A,T8994AAvailable SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAL4DBD-SuUR f.l
Plasmid#112995PurposeP element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genomeDepositorInsertSuUR (SuUR Fly)
UseP insertion vectorAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1_IVT
Plasmid#222342PurposePlasmid for evolved and engineered Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTemplate for in vitro transcriptionPromoterT7 (inactivated)Available SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-Fluc-PGK-Rluc-mCherry
Plasmid#191863PurposeHeat inducible Fluc reporter with constitutive RlucDepositorInsertsFirefly luciferase Fluc
Renilla luciferase Rluc
UseLentiviralTagsP2A-mCherryExpressionMammalianPromoterHSPA7 promoter and PGK promoterAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-eGFP-PGK-mCherry
Plasmid#191862PurposeHeat inducible eGFP reporter with constitutive mCherry markerDepositorInsertseGFP
mCherry
UseLentiviralExpressionMammalianPromoterHSPA7 promoter and PGK promoterAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVMutationCas9 D10APromoterCbhAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189923PurposeAAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVMutationnSaCas9 KKH, TadA ABE8e V106WPromoterEFSAvailable SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHC036 (∆pHG1)
Plasmid#226206PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.DepositorInsertHomology arms for the pHG1 megaplasmid replication region
UseSynthetic BiologyMutation∆helD ∆repA ∆repB ∆parABAvailable SinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDFDuet-nsp7-nsp8
Plasmid#159092PurposeCoexpression construct of Nsp7 with an N-terminal His-tag and Nsp8DepositorInsertsNSP7
NSP8
TagsHisExpressionBacterialPromoterT7Available SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189925PurposeAAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSauriABE8e
UseAAVMutationSauriCas9 D15APromoterEFSAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA251_ Ptrc.x.tetO2::D-HicDH
Plasmid#185456PurposeCDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.DepositorInsertD-HicDH from Lactobacillus paracasei DSM 20008
ExpressionBacterialPromoterPtrc.x.tetO2Available SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI
Plasmid#189924PurposeAAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVMutationSaCas9 D10A, TadA ABE8e V106WPromoterEFSAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189922PurposeAAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e
UseAAVMutationSaCas9 D10APromoterEFSAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only